Abstract
Polycomb-repressive complex 2 (PRC2) facilitates the maintenance and inheritance of chromatin domains repressive to transcription through catalysis of methylation of histone H3 at Lys27 (H3K27me2/3). However, through its EZH2 subunit, PRC2 also binds to nascent transcripts from active genes that are devoid of H3K27me2/3 in embryonic stemcells. Here, biochemical analyses indicated that RNA interaction inhibits SET domain-containing proteins, such as PRC2, nonspecifically in vitro. However, CRISPR-mediated truncation of a PRC2-interacting nascent RNA rescued PRC2-mediated deposition of H3K27me2/3. That PRC2 activity is inhibited by interactions with nascent transcripts supports a model in which PRC2 can only mark for repression those genes silenced by transcriptional repressors.
Original language | English (US) |
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Pages (from-to) | 1983-1988 |
Number of pages | 6 |
Journal | Genes and Development |
Volume | 28 |
Issue number | 18 |
DOIs | |
State | Published - Sep 15 2014 |
Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2014 Kaneko et al.
Keywords
- Chromatin
- EZH2
- JARID2
- PRC2
- ncRNA