Abstract
The aim of this work was to know whether naringin (NA) could prevent the bone complications in a model of streptozotocin (STZ) induced diabetes. Rats were divided in: 1) controls, 2) STZ-rats, 3) STZ-rats treated with 40 mg NA/kg, and 4) STZ-rats treated with 80 mg NA/kg. BMD and BMC were performed by DEXA. Bone histomorphometry and histology as well as TRAP staining were done in tibia. Osteocalcin (OCN) was determined in bone and serum. Glutathione content and SOD and catalase activities were assayed in bone marrow from femur. The data showed that NA80 increased the BMD and BMC from the long bones of STZ-rats. Both NA40 and NA80 normalized the trabecular number and the trabecular separations. An increase in the number of adipocytes and TRAP(+) cells in tibia from STZ-rats was blocked by NA. NA40 treatment increased the number of OCN(+) cells, but only the NA80 treatment allowed to reach the control values. NA normalized the SOD and catalase activities in bone marrow of femur from STZ-rats. In conclusion, NA avoids alterations in the physical properties and microstructure of bone from STZ-rats probably by stimulation of osteoblastogenesis, inhibition of the osteoclastogenesis and adipogenesis via blocking the oxidative stress.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 56-63 |
| Number of pages | 8 |
| Journal | Archives of Biochemistry and Biophysics |
| Volume | 637 |
| DOIs | |
| State | Published - Jan 1 2018 |
| Externally published | Yes |
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Keywords
- Adipocytes
- Bone mineral density
- Naringin
- Oxidative stress
- TRAP(+) cells
- Type 1 D.m.
PubMed: MeSH publication types
- Journal Article
- Research Support, U.S. Gov't, Non-P.H.S.
- Research Support, Non-U.S. Gov't
Cite this
Naringin prevents bone loss in a rat model of type 1 Diabetes mellitus. / Rivoira, M.; Rodríguez, V.; Picotto, G.; Battaglino, R.; Tolosa de Talamoni, N.
In: Archives of Biochemistry and Biophysics, Vol. 637, 01.01.2018, p. 56-63.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Naringin prevents bone loss in a rat model of type 1 Diabetes mellitus
AU - Rivoira, M.
AU - Rodríguez, V.
AU - Picotto, G.
AU - Battaglino, R.
AU - Tolosa de Talamoni, N.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - The aim of this work was to know whether naringin (NA) could prevent the bone complications in a model of streptozotocin (STZ) induced diabetes. Rats were divided in: 1) controls, 2) STZ-rats, 3) STZ-rats treated with 40 mg NA/kg, and 4) STZ-rats treated with 80 mg NA/kg. BMD and BMC were performed by DEXA. Bone histomorphometry and histology as well as TRAP staining were done in tibia. Osteocalcin (OCN) was determined in bone and serum. Glutathione content and SOD and catalase activities were assayed in bone marrow from femur. The data showed that NA80 increased the BMD and BMC from the long bones of STZ-rats. Both NA40 and NA80 normalized the trabecular number and the trabecular separations. An increase in the number of adipocytes and TRAP(+) cells in tibia from STZ-rats was blocked by NA. NA40 treatment increased the number of OCN(+) cells, but only the NA80 treatment allowed to reach the control values. NA normalized the SOD and catalase activities in bone marrow of femur from STZ-rats. In conclusion, NA avoids alterations in the physical properties and microstructure of bone from STZ-rats probably by stimulation of osteoblastogenesis, inhibition of the osteoclastogenesis and adipogenesis via blocking the oxidative stress.
AB - The aim of this work was to know whether naringin (NA) could prevent the bone complications in a model of streptozotocin (STZ) induced diabetes. Rats were divided in: 1) controls, 2) STZ-rats, 3) STZ-rats treated with 40 mg NA/kg, and 4) STZ-rats treated with 80 mg NA/kg. BMD and BMC were performed by DEXA. Bone histomorphometry and histology as well as TRAP staining were done in tibia. Osteocalcin (OCN) was determined in bone and serum. Glutathione content and SOD and catalase activities were assayed in bone marrow from femur. The data showed that NA80 increased the BMD and BMC from the long bones of STZ-rats. Both NA40 and NA80 normalized the trabecular number and the trabecular separations. An increase in the number of adipocytes and TRAP(+) cells in tibia from STZ-rats was blocked by NA. NA40 treatment increased the number of OCN(+) cells, but only the NA80 treatment allowed to reach the control values. NA normalized the SOD and catalase activities in bone marrow of femur from STZ-rats. In conclusion, NA avoids alterations in the physical properties and microstructure of bone from STZ-rats probably by stimulation of osteoblastogenesis, inhibition of the osteoclastogenesis and adipogenesis via blocking the oxidative stress.
KW - Adipocytes
KW - Bone mineral density
KW - Naringin
KW - Oxidative stress
KW - TRAP(+) cells
KW - Type 1 D.m.
UR - http://www.scopus.com/inward/record.url?scp=85037714203&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85037714203&partnerID=8YFLogxK
U2 - 10.1016/j.abb.2017.12.001
DO - 10.1016/j.abb.2017.12.001
M3 - Article
C2 - 29208404
AN - SCOPUS:85037714203
VL - 637
SP - 56
EP - 63
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
ER -