Nanoscale patterning of membrane-bound proteins formed through curvature-induced partitioning of phase-specific receptor lipids

Maria O Ogunyankin Marquez, Dale L. Huber, Darryl Y. Sasaki, Marjorie L. Longo

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

This work describes a technique for forming high-density arrays and patterns of membrane-bound proteins through binding to a curvature-organized compositional pattern of metal-chelating lipids (Cu2+-DOIDA or Cu2+-DSIDA). In this bottom-up approach, the underlying support is an e-beam formed, square lattice pattern of hemispheres. This curvature pattern sorts Cu2+-DOIDA to the 200 nm hemispherical lattice sites of a 600 nm × 600 nm unit cell in Ld - Lo phase separated lipid multibilayers. Binding of histidine-tagged green fluorescent protein (His-GFP) creates a high density array of His-GFP-bound pixels localized to the square lattice sites. In comparison, the negative pixel pattern is created by sorting Cu2+-DSIDA in Ld - Lβ′ phase separated lipid multibilayers to the flat grid between the lattice sites followed by binding to His-GFP. Lattice defects in the His-GFP pattern lead to interesting features such as pattern circularity. We also observe defect-free arrays of His-GFP that demonstrate perfect arrays can be formed by this method suggesting the possibility of using this approach for the localization of various active molecules to form protein, DNA, or optically active molecular arrays.

Original languageEnglish (US)
Pages (from-to)6109-6115
Number of pages7
JournalLangmuir
Volume29
Issue number20
DOIs
StatePublished - May 21 2013

Fingerprint Dive into the research topics of 'Nanoscale patterning of membrane-bound proteins formed through curvature-induced partitioning of phase-specific receptor lipids'. Together they form a unique fingerprint.

Cite this