TY - JOUR
T1 - Nanoporous silica glass for the immobilization of interactive enzyme systems.
AU - Buthe, Andreas
AU - Wu, Songtao
AU - Wang, Ping
PY - 2011
Y1 - 2011
N2 - Recent pursuit on utilization of nanoscale materials has manifested a variety of configurations of highly efficient enzymic biocatalyst systems for biotechnological applications. Nanoscale structures are particularly powerful in effecting multienzyme biocatalysis. Inherent properties of nanomaterials--primarily, the high surface area to volume ratio and atomic scale 3D configurations--enable higher enzyme loadings, microenvironment control surrounding enzyme molecules, regulation on mass transfer, and protein structural stabilization of the biocatalyst as compared to traditional immobilization systems. This chapter introduces one versatile nanoscale immobilization method via details demonstrated using the case of nanoporous silica glass (30 nm diameter) for the concomitant incorporation of lactate dehydrogenase (LDH), glucose dehydrogenase (GDH), and the cofactor (NADH).
AB - Recent pursuit on utilization of nanoscale materials has manifested a variety of configurations of highly efficient enzymic biocatalyst systems for biotechnological applications. Nanoscale structures are particularly powerful in effecting multienzyme biocatalysis. Inherent properties of nanomaterials--primarily, the high surface area to volume ratio and atomic scale 3D configurations--enable higher enzyme loadings, microenvironment control surrounding enzyme molecules, regulation on mass transfer, and protein structural stabilization of the biocatalyst as compared to traditional immobilization systems. This chapter introduces one versatile nanoscale immobilization method via details demonstrated using the case of nanoporous silica glass (30 nm diameter) for the concomitant incorporation of lactate dehydrogenase (LDH), glucose dehydrogenase (GDH), and the cofactor (NADH).
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U2 - 10.1007/978-1-60761-895-9_5
DO - 10.1007/978-1-60761-895-9_5
M3 - Article
C2 - 20865387
AN - SCOPUS:79952198691
SN - 1064-3745
VL - 679
SP - 37
EP - 48
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -