Nanoparticle-Mediated Photodynamic Therapy for Mixed Biofilms

Marina Usacheva, Buddhadev Layek, Saif S. Rahman Nirzhor, Swayam Prabha

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

Wounds associated with diabetes are difficult to heal and often stay unhealed, leading to higher morbidity and mortality in the diabetic population. A major barrier to the successful healing of chronic diabetic wounds is the presence of biofilm-mediated infections. In recent years, photodynamic therapy (PDT) has emerged as a promising treatment modality for the management of chronic wounds. The objective of the present study was to evaluate the effectiveness of toluidine blue- (TB-) loaded nanoparticle-mediated PDT in eradicating antibiotic-resistant polymicrobial biofilms of Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus in an in vitro model. Dioctyl sodium sulfosuccinate (aerosol OT, AOT)-alginate nanoparticles with high TB loading (10.8 ± 2.2 %) were formulated using a double emulsification cross-linking method. TB nanoparticles induced effective killing of planktonic P. aeruginosa (3.5 log10 CFU) and S. aureus (>5 log10 CFU) and their combined biofilms (2.8 log10 CFU for P. aeruginosa versus 3.4 log10 CFU for S. aureus). While P. aeruginosa biofilm was more resistant when compared to that of S. aureus, our results demonstrated effective eradication of complex biofilms of dual bacterial strains in vitro.

Original languageEnglish (US)
Article number4752894
JournalJournal of Nanomaterials
Volume2016
DOIs
StatePublished - 2016

Bibliographical note

Publisher Copyright:
© 2016 Marina Usacheva et al.

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