Nanohole Array-Directed Trapping of Mammalian Mitochondria Enabling Single Organelle Analysis

Shailabh Kumar, Gregory G. Wolken, Nathan J. Wittenberg, Edgar A. Arriaga, Sang Hyun Oh

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


We present periodic nanohole arrays fabricated in free-standing metal-coated nitride films as a platform for trapping and analyzing single organelles. When a microliter-scale droplet containing mitochondria is dispensed above the nanohole array, the combination of evaporation and capillary flow directs individual mitochondria to the nanoholes. Mammalian mitochondria arrays were rapidly formed on chip using this technique without any surface modification steps, microfluidic interconnects, or external power sources. The trapped mitochondria were depolarized on chip using an ionophore with results showing that the organelle viability and behavior were preserved during the on-chip assembly process. Fluorescence signal related to mitochondrial membrane potential was obtained from single mitochondria trapped in individual nanoholes revealing statistical differences between the behavior of polarized vs depolarized mammalian mitochondria. This technique provides a fast and stable route for droplet-based directed localization of organelles-on-a-chip with minimal limitations and complexity, as well as promotes integration with other optical or electrochemical detection techniques.

Original languageEnglish (US)
Pages (from-to)11973-11977
Number of pages5
JournalAnalytical Chemistry
Issue number24
StatePublished - Dec 15 2015

Bibliographical note

Publisher Copyright:
© 2015 American Chemical Society.


Dive into the research topics of 'Nanohole Array-Directed Trapping of Mammalian Mitochondria Enabling Single Organelle Analysis'. Together they form a unique fingerprint.

Cite this