Nanoerythrosomes tailoring: Lipid induced protein scaffolding in ghost membrane derived vesicles

Róbert Deák; Judith Mihály; Imola Cs Szigyártó; Tamás Beke-Somfai; Lilla Turiák; László Drahos; András Wacha; Attila Bóta; Zoltán Varga

doi:10.1016/j.msec.2019.110428

Nanoerythrosomes tailoring: Lipid induced protein scaffolding in ghost membrane derived vesicles

Róbert Deák
, Judith Mihály
, Imola Cs Szigyártó
, Tamás Beke-Somfai
, Lilla Turiák
, László Drahos
, András Wacha
, Attila Bóta
, Zoltán Varga

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

A peculiar polygonal protein scaffolding that resembles to spectrin-based skeleton of red blood cells can be reconstructed on the outer surface of vesicle-like nanoerythrosomes. The approximately 130 nm sized nanoerythrosomes are produced from red blood cell ghosts by addition of phospholipids (dipalmitoylphosphatidylcholine, DPPC). The scaffolding, constructed from the structural proteins of the cell membrane skeleton, covers the whole object resulting an enhanced stiffness. The protein pattern of the scaffolding is thermosensitive, reversible transformable in the biologically relevant temperature range. When the lipid additive is changed from DPPC to lysophospholipid (LPC), the protein network/scaffolding ceases to exist. By the variation of lipid type and ratio, a tailoring of the nanoerythrosomes can be achieved. During the tailoring process nanoerythrosomes or micelles, in a wide size range from 200 to 30 nm, are produced.

Original language	English (US)
Article number	110428
Journal	Materials Science and Engineering C
Volume	109
DOIs	https://doi.org/10.1016/j.msec.2019.110428
State	Published - Apr 2020
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the National Research Development and Innovation Office (Hungary) under grant numbers PD 121326 and NVKP_16-1-2016-0007 , HunProtExc (A. Bóta) and Protein research program of Hungarian Academy of Sciences (MEDinPROT, A. Bóta). Z. Varga was supported by the János Bolyai Research Fellowship of the Hungarian Academy of Sciences . We thank Teréz Kiss for freeze fracturing and Ferenc Zsila for reviewing polarized light spectroscopy measurements.

Publisher Copyright:
© 2019

Keywords

Ghost
Nanoerythrosome
Protein - lipid interactions
Protein network
Protein scaffolding

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10.1016/j.msec.2019.110428

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@article{35d43696b24e4d31ab6fb3f34b1d9fb9,

title = "Nanoerythrosomes tailoring: Lipid induced protein scaffolding in ghost membrane derived vesicles",

abstract = "A peculiar polygonal protein scaffolding that resembles to spectrin-based skeleton of red blood cells can be reconstructed on the outer surface of vesicle-like nanoerythrosomes. The approximately 130 nm sized nanoerythrosomes are produced from red blood cell ghosts by addition of phospholipids (dipalmitoylphosphatidylcholine, DPPC). The scaffolding, constructed from the structural proteins of the cell membrane skeleton, covers the whole object resulting an enhanced stiffness. The protein pattern of the scaffolding is thermosensitive, reversible transformable in the biologically relevant temperature range. When the lipid additive is changed from DPPC to lysophospholipid (LPC), the protein network/scaffolding ceases to exist. By the variation of lipid type and ratio, a tailoring of the nanoerythrosomes can be achieved. During the tailoring process nanoerythrosomes or micelles, in a wide size range from 200 to 30 nm, are produced.",

keywords = "Ghost, Nanoerythrosome, Protein - lipid interactions, Protein network, Protein scaffolding",

author = "R{\'o}bert De{\'a}k and Judith Mih{\'a}ly and Szigy{\'a}rt{\'o}, \{Imola Cs\} and Tam{\'a}s Beke-Somfai and Lilla Turi{\'a}k and L{\'a}szl{\'o} Drahos and Andr{\'a}s Wacha and Attila B{\'o}ta and Zolt{\'a}n Varga",

note = "Funding Information: This work was supported by the National Research Development and Innovation Office (Hungary) under grant numbers PD 121326 and NVKP\_16-1-2016-0007 , HunProtExc (A. B{\'o}ta) and Protein research program of Hungarian Academy of Sciences (MEDinPROT, A. B{\'o}ta). Z. Varga was supported by the J{\'a}nos Bolyai Research Fellowship of the Hungarian Academy of Sciences . We thank Ter{\'e}z Kiss for freeze fracturing and Ferenc Zsila for reviewing polarized light spectroscopy measurements. Publisher Copyright: {\textcopyright} 2019",

year = "2020",

month = apr,

doi = "10.1016/j.msec.2019.110428",

language = "English (US)",

volume = "109",

journal = "Materials Science and Engineering C",

issn = "0928-4931",

publisher = "Elsevier Limited",

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TY - JOUR

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T2 - Lipid induced protein scaffolding in ghost membrane derived vesicles

AU - Deák, Róbert

AU - Mihály, Judith

AU - Szigyártó, Imola Cs

AU - Beke-Somfai, Tamás

AU - Turiák, Lilla

AU - Drahos, László

AU - Wacha, András

AU - Bóta, Attila

AU - Varga, Zoltán

N1 - Funding Information: This work was supported by the National Research Development and Innovation Office (Hungary) under grant numbers PD 121326 and NVKP_16-1-2016-0007 , HunProtExc (A. Bóta) and Protein research program of Hungarian Academy of Sciences (MEDinPROT, A. Bóta). Z. Varga was supported by the János Bolyai Research Fellowship of the Hungarian Academy of Sciences . We thank Teréz Kiss for freeze fracturing and Ferenc Zsila for reviewing polarized light spectroscopy measurements. Publisher Copyright: © 2019

PY - 2020/4

Y1 - 2020/4

N2 - A peculiar polygonal protein scaffolding that resembles to spectrin-based skeleton of red blood cells can be reconstructed on the outer surface of vesicle-like nanoerythrosomes. The approximately 130 nm sized nanoerythrosomes are produced from red blood cell ghosts by addition of phospholipids (dipalmitoylphosphatidylcholine, DPPC). The scaffolding, constructed from the structural proteins of the cell membrane skeleton, covers the whole object resulting an enhanced stiffness. The protein pattern of the scaffolding is thermosensitive, reversible transformable in the biologically relevant temperature range. When the lipid additive is changed from DPPC to lysophospholipid (LPC), the protein network/scaffolding ceases to exist. By the variation of lipid type and ratio, a tailoring of the nanoerythrosomes can be achieved. During the tailoring process nanoerythrosomes or micelles, in a wide size range from 200 to 30 nm, are produced.

AB - A peculiar polygonal protein scaffolding that resembles to spectrin-based skeleton of red blood cells can be reconstructed on the outer surface of vesicle-like nanoerythrosomes. The approximately 130 nm sized nanoerythrosomes are produced from red blood cell ghosts by addition of phospholipids (dipalmitoylphosphatidylcholine, DPPC). The scaffolding, constructed from the structural proteins of the cell membrane skeleton, covers the whole object resulting an enhanced stiffness. The protein pattern of the scaffolding is thermosensitive, reversible transformable in the biologically relevant temperature range. When the lipid additive is changed from DPPC to lysophospholipid (LPC), the protein network/scaffolding ceases to exist. By the variation of lipid type and ratio, a tailoring of the nanoerythrosomes can be achieved. During the tailoring process nanoerythrosomes or micelles, in a wide size range from 200 to 30 nm, are produced.

KW - Ghost

KW - Nanoerythrosome

KW - Protein - lipid interactions

KW - Protein network

KW - Protein scaffolding

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UR - https://www.scopus.com/pages/publications/85076701398#tab=citedBy

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JO - Materials Science and Engineering C

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ER -

Nanoerythrosomes tailoring: Lipid induced protein scaffolding in ghost membrane derived vesicles

Abstract

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