Abstract
The use of nano-LC MALDI-QTOF-MS to identify and quantify ICAT labeled protein mixtures was analyzed. Proteins expressed by a cell under two different physiological conditions were labeled with ICAT reagent on their crysteinyl residues. After washing, the labeled peptides were eluted from the avidin column and separated by reversed phase nano-LC. Results showed that the technique has great potential for bypassing the 2D gels and the study of protein expression levels from different cell states.
| Original language | English (US) |
|---|---|
| Title of host publication | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
| Pages | 289-290 |
| Number of pages | 2 |
| State | Published - Dec 1 2002 |
| Event | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002 |
Other
| Other | Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics |
|---|---|
| Country/Territory | United States |
| City | Orlando, FL |
| Period | 6/2/02 → 6/6/02 |