Nano-LC MALDI-QTOF-MS for the analysis of complex ICAT labeled protein mixtures

Remco Van Soest, Emmanuel Varesio, Irina Braaf-Dragan, Hookeun Lee, Timothy Griffin, Ruedi Aebersold

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The use of nano-LC MALDI-QTOF-MS to identify and quantify ICAT labeled protein mixtures was analyzed. Proteins expressed by a cell under two different physiological conditions were labeled with ICAT reagent on their crysteinyl residues. After washing, the labeled peptides were eluted from the avidin column and separated by reversed phase nano-LC. Results showed that the technique has great potential for bypassing the 2D gels and the study of protein expression levels from different cell states.

Original languageEnglish (US)
Title of host publicationProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages289-290
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

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Proteins
Avidin
Washing
Gels
Peptides

Cite this

Van Soest, R., Varesio, E., Braaf-Dragan, I., Lee, H., Griffin, T., & Aebersold, R. (2002). Nano-LC MALDI-QTOF-MS for the analysis of complex ICAT labeled protein mixtures. In Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 289-290)

Nano-LC MALDI-QTOF-MS for the analysis of complex ICAT labeled protein mixtures. / Van Soest, Remco; Varesio, Emmanuel; Braaf-Dragan, Irina; Lee, Hookeun; Griffin, Timothy; Aebersold, Ruedi.

Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 289-290.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Van Soest, R, Varesio, E, Braaf-Dragan, I, Lee, H, Griffin, T & Aebersold, R 2002, Nano-LC MALDI-QTOF-MS for the analysis of complex ICAT labeled protein mixtures. in Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 289-290, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Van Soest R, Varesio E, Braaf-Dragan I, Lee H, Griffin T, Aebersold R. Nano-LC MALDI-QTOF-MS for the analysis of complex ICAT labeled protein mixtures. In Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 289-290
Van Soest, Remco ; Varesio, Emmanuel ; Braaf-Dragan, Irina ; Lee, Hookeun ; Griffin, Timothy ; Aebersold, Ruedi. / Nano-LC MALDI-QTOF-MS for the analysis of complex ICAT labeled protein mixtures. Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 289-290
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