Nano-lamp arrays for multiplex microfluidic SPR biosensing

Antoine Lesuffleur; Hyungsoon Im; Nathan C. Lindquist; Kwan Seop Lim; Sang Hyun Oh

Nano-lamp arrays for multiplex microfluidic SPR biosensing

Antoine Lesuffleur, Hyungsoon Im, Nathan C. Lindquist, Kwan Seop Lim, Sang Hyun Oh

Electrical and Computer Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Surface Plasmon Resonance imaging (SPRi) is a powerful technique for highthroughput, real-time, label-free characterization of molecular interactions. The recent discovery of extraordinary optical transmission through periodic nanohole arrays in a metallic film [1] led to many novel applications, including SPR biosensing. Here, we demonstrate integrating several nanohole arrays for multiplex SPR detection using a laser source and a CCD camera for imaging transmission through each array in a real-time manner. Binding kinetics of self-assembled monolayer (SAM) adsorption onto the gold surface and specific interactions between biotin and streptavidin were imaged in real-time using the nanoscale LAserilluminated Metal Pores (nano-LAMP) system.

Original language	English (US)
Title of host publication	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
Publisher	Chemical and Biological Microsystems Society
Pages	955-957
Number of pages	3
State	Published - Jan 1 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

Keywords

Label-free biosensing
Nanohole array
Surface plasmon resonance

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Nano-lamp arrays for multiplex microfluidic SPR biosensing. / Lesuffleur, Antoine; Im, Hyungsoon; Lindquist, Nathan C. et al.
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. p. 955-957.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lesuffleur, A, Im, H, Lindquist, NC, Lim, KS & Oh, SH 2008, Nano-lamp arrays for multiplex microfluidic SPR biosensing. in 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, pp. 955-957, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 10/12/08.

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title = "Nano-lamp arrays for multiplex microfluidic SPR biosensing",

abstract = "Surface Plasmon Resonance imaging (SPRi) is a powerful technique for highthroughput, real-time, label-free characterization of molecular interactions. The recent discovery of extraordinary optical transmission through periodic nanohole arrays in a metallic film [1] led to many novel applications, including SPR biosensing. Here, we demonstrate integrating several nanohole arrays for multiplex SPR detection using a laser source and a CCD camera for imaging transmission through each array in a real-time manner. Binding kinetics of self-assembled monolayer (SAM) adsorption onto the gold surface and specific interactions between biotin and streptavidin were imaged in real-time using the nanoscale LAserilluminated Metal Pores (nano-LAMP) system.",

keywords = "Label-free biosensing, Nanohole array, Surface plasmon resonance",

author = "Antoine Lesuffleur and Hyungsoon Im and Lindquist, {Nathan C.} and Lim, {Kwan Seop} and Oh, {Sang Hyun}",

year = "2008",

month = jan,

day = "1",

language = "English (US)",

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booktitle = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference",

publisher = "Chemical and Biological Microsystems Society",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

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T1 - Nano-lamp arrays for multiplex microfluidic SPR biosensing

AU - Lesuffleur, Antoine

AU - Im, Hyungsoon

AU - Lindquist, Nathan C.

AU - Lim, Kwan Seop

AU - Oh, Sang Hyun

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Surface Plasmon Resonance imaging (SPRi) is a powerful technique for highthroughput, real-time, label-free characterization of molecular interactions. The recent discovery of extraordinary optical transmission through periodic nanohole arrays in a metallic film [1] led to many novel applications, including SPR biosensing. Here, we demonstrate integrating several nanohole arrays for multiplex SPR detection using a laser source and a CCD camera for imaging transmission through each array in a real-time manner. Binding kinetics of self-assembled monolayer (SAM) adsorption onto the gold surface and specific interactions between biotin and streptavidin were imaged in real-time using the nanoscale LAserilluminated Metal Pores (nano-LAMP) system.

AB - Surface Plasmon Resonance imaging (SPRi) is a powerful technique for highthroughput, real-time, label-free characterization of molecular interactions. The recent discovery of extraordinary optical transmission through periodic nanohole arrays in a metallic film [1] led to many novel applications, including SPR biosensing. Here, we demonstrate integrating several nanohole arrays for multiplex SPR detection using a laser source and a CCD camera for imaging transmission through each array in a real-time manner. Binding kinetics of self-assembled monolayer (SAM) adsorption onto the gold surface and specific interactions between biotin and streptavidin were imaged in real-time using the nanoscale LAserilluminated Metal Pores (nano-LAMP) system.

KW - Label-free biosensing

KW - Nanohole array

KW - Surface plasmon resonance

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T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Nano-lamp arrays for multiplex microfluidic SPR biosensing

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