Naive B Cells with High-Avidity Germline-Encoded Antigen Receptors Produce Persistent IgM + and Transient IgG + Memory B Cells

Kathryn A Pape, Robert W. Maul, Dileepan T, Amanda Schmidt Paustian, Patricia J. Gearhart, Marc Jenkins

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Although immune memory often lasts for life, this is not the case for certain vaccines in some individuals. We sought a mechanism for this phenomenon by studying B cell responses to phycoerythrin (PE). PE immunization of mouse strains with Igh b immunoglobulin (Ig) variable heavy chain (V H ) genes elicited affinity-matured switched Ig memory B cells that declined with time, while the comparable population from an Igh a strain was numerically stable. Igh b strains had larger numbers of PE-specific naive B cells and generated smaller germinal center responses and larger numbers of IgM memory cells than the Igh a strain. The properties of PE-specific B cells in Igh b mice correlated with usage of a single V H that afforded high-affinity PE binding in its germline form. These results suggest that some individuals may be genetically predisposed to generate non-canonical memory B cell responses to certain antigens because of avid antigen binding via germline-encoded V H elements. Immunity induced by certain vaccines declines over time. By studying B cell responses to phycoerythrin, Pape et al. find that memory B cells can be short-lived when generated from precursors that experience unusually strong early signals through their un-mutated antigen receptors.

Original languageEnglish (US)
Pages (from-to)1135-1143.e4
JournalImmunity
Volume48
Issue number6
DOIs
StatePublished - Jun 19 2018

Fingerprint

Antigen Receptors
Phycoerythrin
Immunoglobulin M
B-Lymphocytes
Immunoglobulin G
Vaccines
Immunoglobulin Heavy Chain Genes
Antigens
Germinal Center
Immunity
Immunization
Population

Keywords

  • V
  • memory B cell
  • naive B cell
  • repertoire

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

Cite this

Naive B Cells with High-Avidity Germline-Encoded Antigen Receptors Produce Persistent IgM + and Transient IgG + Memory B Cells. / Pape, Kathryn A; Maul, Robert W.; T, Dileepan; Paustian, Amanda Schmidt; Gearhart, Patricia J.; Jenkins, Marc.

In: Immunity, Vol. 48, No. 6, 19.06.2018, p. 1135-1143.e4.

Research output: Contribution to journalArticle

Pape, Kathryn A ; Maul, Robert W. ; T, Dileepan ; Paustian, Amanda Schmidt ; Gearhart, Patricia J. ; Jenkins, Marc. / Naive B Cells with High-Avidity Germline-Encoded Antigen Receptors Produce Persistent IgM + and Transient IgG + Memory B Cells. In: Immunity. 2018 ; Vol. 48, No. 6. pp. 1135-1143.e4.
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