N-acetyl-L-cysteine protects endothelial cells but not L929 tumor cells from tumor necrosis factor-α-mediated cytotoxicity

Henning Schröder, Scott Warren, Mario J. Bargetzi, Suzy V. Torti, Frank M. Torti

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The effect of N-acetyl-L-cysteine on the cytotoxicity of tumor necrosis factor-α was investigated in cultured bovine pulmonary artery endothelial cells and L929 mouse tumor cells. In endothelial cells, a 72-h incubation with tumor necrosis factor-α (100 ng/ml) reduced the number of viable cells to 27% of control. Simultaneous incubation with N-acetyl-Lcysteine (0.5–5 mmol/l) protected endothelial cells from tumor necrosis factor-α-mediated cytotoxicity and increased viability in a concentration-dependent fashion to 69% of control. Under the same conditions, a 72-h incubation with tumor necrosis factor-α (100 ng/ml) reduced the number of viable L929 tumor cells to 31% of control. However, this cytotoxic response remained unaltered in the presence of N-acetyl-Lrcysteine (0.5–5 mmol/l). Similar results were obtained when using a lower concentration of tumor necrosis factor-α (50 ng/ml). These findings demonstrate protection from tumor necrosis factor-α-mediated toxicity by N-acetyl-L-cysteine in endothelial cells but not in a tumor cell line. It is concluded that N-acetyl-L-cysteine might serve as a therapeutic agent to limit the vascular toxicity of tumor necrosis factor-α without affecting its antineoplastic activity.

Original languageEnglish (US)
Pages (from-to)664-666
Number of pages3
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume347
Issue number6
DOIs
StatePublished - Jun 1993

Keywords

  • Cytotoxicity
  • Endothelial cells
  • L929 tumor cells
  • N-acetyl- L-eysteine
  • Tumor necrosis factor

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