Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf

Sung Keun Jung; Ki Won Lee; Ho Young Kim; Mi Hyun Oh; Sanguine Byun; Sung Hwan Lim; Yong Seok Heo; Nam Joo Kang; Ann M. Bode; Zigang Dong; Hyong Joo Lee

doi:10.1016/j.bcp.2010.01.004

Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf

Sung Keun Jung, Ki Won Lee, Ho Young Kim, Mi Hyun Oh, Sanguine Byun, Sung Hwan Lim, Yong Seok Heo, Nam Joo Kang, Ann M. Bode, Zigang Dong, Hyong Joo Lee

The Hormel Institute

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

Chronic exposure to solar ultraviolet (UV) light causes skin photoaging. Many studies have shown that naturally occurring phytochemicals have anti-photoaging effects, but their direct target molecule(s) and mechanism(s) remain unclear. We found that myricetin, a major flavonoid in berries and red wine, inhibited wrinkle formation in mouse skin induced by chronic UVB irradiation (0.18 J/cm², 3 days/week for 15 weeks). Myricetin treatment reduced UVB-induced epidermal thickening of mouse skin and also suppressed UVB-induced matrix metalloproteinase-9 (MMP-9) protein expression and enzyme activity. Myricetin appeared to exert its anti-aging effects by suppressing UVB-induced Raf kinase activity and subsequent attenuation of UVB-induced phosphorylation of MEK and ERK in mouse skin. In vitro and in vivo pull-down assays revealed that myricetin bound with Raf in an ATP-noncompetitive manner. Overall, these results indicate that myricetin exerts potent anti-photoaging activity by regulating MMP-9 expression through the suppression of Raf kinase activity.

Original language	English (US)
Pages (from-to)	1455-1461
Number of pages	7
Journal	Biochemical Pharmacology
Volume	79
Issue number	10
DOIs	https://doi.org/10.1016/j.bcp.2010.01.004
State	Published - May 15 2010

Bibliographical note

Funding Information:
This study was supported by a grant from the BioGreen21 Program (Nos. 20070301-034-027 and 042 ) of the Rural Development Administration; World Class University Program ( R31-2008-00-10056-0 ) and Priority Research Centers Program ( 2009-0093824 ), the National Research Foundation of Korea, the Ministry of Education, Science, and Technology, Republic of Korea. Work was also supported by The Hormel Foundation and NIH grants CA120388 , R37CA081064 , CA077646 , CA027502 , and CA111536 .

Keywords

Anti-wrinkle
Myricetin
Photoaging
Raf
UVB

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10.1016/j.bcp.2010.01.004

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title = "Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf",

abstract = "Chronic exposure to solar ultraviolet (UV) light causes skin photoaging. Many studies have shown that naturally occurring phytochemicals have anti-photoaging effects, but their direct target molecule(s) and mechanism(s) remain unclear. We found that myricetin, a major flavonoid in berries and red wine, inhibited wrinkle formation in mouse skin induced by chronic UVB irradiation (0.18 J/cm2, 3 days/week for 15 weeks). Myricetin treatment reduced UVB-induced epidermal thickening of mouse skin and also suppressed UVB-induced matrix metalloproteinase-9 (MMP-9) protein expression and enzyme activity. Myricetin appeared to exert its anti-aging effects by suppressing UVB-induced Raf kinase activity and subsequent attenuation of UVB-induced phosphorylation of MEK and ERK in mouse skin. In vitro and in vivo pull-down assays revealed that myricetin bound with Raf in an ATP-noncompetitive manner. Overall, these results indicate that myricetin exerts potent anti-photoaging activity by regulating MMP-9 expression through the suppression of Raf kinase activity.",

keywords = "Anti-wrinkle, Myricetin, Photoaging, Raf, UVB",

author = "Jung, {Sung Keun} and Lee, {Ki Won} and Kim, {Ho Young} and Oh, {Mi Hyun} and Sanguine Byun and Lim, {Sung Hwan} and Heo, {Yong Seok} and Kang, {Nam Joo} and Bode, {Ann M.} and Zigang Dong and Lee, {Hyong Joo}",

note = "Funding Information: This study was supported by a grant from the BioGreen21 Program (Nos. 20070301-034-027 and 042 ) of the Rural Development Administration; World Class University Program ( R31-2008-00-10056-0 ) and Priority Research Centers Program ( 2009-0093824 ), the National Research Foundation of Korea, the Ministry of Education, Science, and Technology, Republic of Korea. Work was also supported by The Hormel Foundation and NIH grants CA120388 , R37CA081064 , CA077646 , CA027502 , and CA111536 .",

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doi = "10.1016/j.bcp.2010.01.004",

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AU - Jung, Sung Keun

AU - Lee, Ki Won

AU - Kim, Ho Young

AU - Oh, Mi Hyun

AU - Byun, Sanguine

AU - Lim, Sung Hwan

AU - Heo, Yong Seok

AU - Kang, Nam Joo

AU - Bode, Ann M.

AU - Dong, Zigang

AU - Lee, Hyong Joo

N1 - Funding Information: This study was supported by a grant from the BioGreen21 Program (Nos. 20070301-034-027 and 042 ) of the Rural Development Administration; World Class University Program ( R31-2008-00-10056-0 ) and Priority Research Centers Program ( 2009-0093824 ), the National Research Foundation of Korea, the Ministry of Education, Science, and Technology, Republic of Korea. Work was also supported by The Hormel Foundation and NIH grants CA120388 , R37CA081064 , CA077646 , CA027502 , and CA111536 .

PY - 2010/5/15

Y1 - 2010/5/15

N2 - Chronic exposure to solar ultraviolet (UV) light causes skin photoaging. Many studies have shown that naturally occurring phytochemicals have anti-photoaging effects, but their direct target molecule(s) and mechanism(s) remain unclear. We found that myricetin, a major flavonoid in berries and red wine, inhibited wrinkle formation in mouse skin induced by chronic UVB irradiation (0.18 J/cm2, 3 days/week for 15 weeks). Myricetin treatment reduced UVB-induced epidermal thickening of mouse skin and also suppressed UVB-induced matrix metalloproteinase-9 (MMP-9) protein expression and enzyme activity. Myricetin appeared to exert its anti-aging effects by suppressing UVB-induced Raf kinase activity and subsequent attenuation of UVB-induced phosphorylation of MEK and ERK in mouse skin. In vitro and in vivo pull-down assays revealed that myricetin bound with Raf in an ATP-noncompetitive manner. Overall, these results indicate that myricetin exerts potent anti-photoaging activity by regulating MMP-9 expression through the suppression of Raf kinase activity.

AB - Chronic exposure to solar ultraviolet (UV) light causes skin photoaging. Many studies have shown that naturally occurring phytochemicals have anti-photoaging effects, but their direct target molecule(s) and mechanism(s) remain unclear. We found that myricetin, a major flavonoid in berries and red wine, inhibited wrinkle formation in mouse skin induced by chronic UVB irradiation (0.18 J/cm2, 3 days/week for 15 weeks). Myricetin treatment reduced UVB-induced epidermal thickening of mouse skin and also suppressed UVB-induced matrix metalloproteinase-9 (MMP-9) protein expression and enzyme activity. Myricetin appeared to exert its anti-aging effects by suppressing UVB-induced Raf kinase activity and subsequent attenuation of UVB-induced phosphorylation of MEK and ERK in mouse skin. In vitro and in vivo pull-down assays revealed that myricetin bound with Raf in an ATP-noncompetitive manner. Overall, these results indicate that myricetin exerts potent anti-photoaging activity by regulating MMP-9 expression through the suppression of Raf kinase activity.

KW - Anti-wrinkle

KW - Myricetin

KW - Photoaging

KW - Raf

KW - UVB

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ER -

Myricetin suppresses UVB-induced wrinkle formation and MMP-9 expression by inhibiting Raf

Abstract

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Keywords

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