Myogenic progenitors from mouse pluripotent stem cells for muscle regeneration

Alessandro Magli, Tania Incitti, Rita C.R. Perlingeiro

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations


Muscle homeostasis is maintained by resident stem cells which, in both pathologic and non-pathologic conditions, are able to repair or generate new muscle fibers. Although muscle stem cells have tremendous regenerative potential, their application in cell therapy protocols is prevented by several restrictions, including the limited ability to grow ex vivo. Since pluripotent stem cells have the unique potential to both selfrenew and expand almost indefinitely, they have become an attractive source of progenitors for regenerative medicine studies. Our lab has demonstrated that embryonic stem cell (ES)-derived myogenic progenitors retain the ability to repair existing muscle fibers and contribute to the pool of resident stem cells. Because of their relevance in both cell therapy and disease modeling, in this chapter we describe the protocol to derive myogenic progenitors from murine ES cells followed by their intramuscular delivery in a murine muscular dystrophy model.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages18
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2016.


  • Cell therapy
  • ES and iPS cells
  • Muscle differentiation
  • Myogenic progenitors
  • Pax3
  • Pax7
  • Pluripotent
  • Skeletal muscle
  • Transplantation


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