Mycoplasma hyopneumoniae genetic variability within a swine operation

Lucina Galina Pantoja, Kalie Pettit, Lucas F. Dos Santos, Rick Tubbs, Maria Pieters

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

The objective of our study was to characterize the Mycoplasma hyopneumoniae genetic diversity within a swine operation comingling weaned pigs. Bronchial swabs and tracheal aspirates were collected from 3 nursery-to-finish farms. During the finishing production stages, samples were obtained from mortalities and from live coughing pigs in rooms where mortality was not observed. A total of 105 samples were examined by a M. hyopneumoniae real-time polymerase chain reaction and subjected to genetic typing using a multilocus variable number tandem repeat analysis (MLVA) assay. The MLVA was used to identify genetic variants based on the number of repeats in 2 variable number tandem repeats loci, namely P97 and P146, thought to mediate adherence of M. hyopneumoniae to swine cilia. Four distinguishable M. hyopneumoniae variants were identified: MVLA variants 9-15, 11-21, 9-21, and 7-15. Variant 9-15 was the most prevalent, observed in 79% of rooms, and detected on all 3 farms. Variant 11-21 was present in 37% of the rooms on 2 of the 3 farms. Only one 9-21 variant was identified in 1 farm, and all samples of variant 7-15 were recovered from another farm. Based on the low prevalence and limited geographic distribution of the last 2 variants, it is hypothesized that they might be the result of in-situ recombination. All variants detected in this investigation appeared to belong to 3 clusters. Overall, a limited number of variants and clusters were identified in a system that comingles pigs from different sources, suggesting limited M. hyopneumoniae genetic variation within commercial swine production environments.

Original languageEnglish (US)
Pages (from-to)175-179
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume28
Issue number2
DOIs
StatePublished - Mar 1 2016

Bibliographical note

Publisher Copyright:
© 2016, © 2016 The Author(s).

Keywords

  • Genetic variability
  • MLVA typing
  • Mycoplasma hyopneumoniae
  • swine

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