Mycobacterium sarraceniae sp. Nov. and mycobacterium helvum sp. nov., isolated from the pitcher plant sarracenia purpurea

Phuong M. Tran, John L Dahl

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Several fast-to intermediate-growing, acid-fast, scotochromogenic bacteria were isolated from Sarracenia purpurea pitcher waters in Minnesota sphagnum peat bogs. Two strains (DL734Tand DL739T) were among these isolates. On the basis of 16S rRNA gene sequences, the phylogenetic positions of both strains is in the genus Mycobacterium with no obvious relation to any characterized type strains of mycobacteria. Phenotypic characterization revealed that neither strain was similar to the type strains of known species of the genus Mycobacterium in the collective properties of growth, pigmentation or fatty acid composition. Strain DL734Tgrew at temperatures between 28 and 32 °C, was positive for 3-day arylsulfatase production, and was negative for Tween 80 hydrolysis, urease and nitrate reduction. Strain DL739Tgrew at temperatures between 28 and 37 °C, and was positive for Tween 80 hydrolysis, urea, nitrate reduction and 3-day arylsulfatase production. Both strains were catalase-negative while only DL739Tgrew with 5%NaCl. Fatty acid methyl ester profiles were unique for each strain. DL739Tshowed an ability to survive at 8 °C with little to no cellular replication and is thus considered to be psychrotolerant. Therefore, strains DL734Tand DL739Trepresent two novel species of the genus Mycobacterium with the proposed names Mycobacterium sarraceniae sp. nov. and Mycobacterium helvum sp. nov., respectively. The type strains are DL734T(=JCM 30395T=NCCB 100519T) and DL739T(=JCM 30396T=NCCB 100520T), respectively.

Original languageEnglish (US)
Article number001377
Pages (from-to)4480-4485
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume66
Issue number11
DOIs
StatePublished - Nov 2016

Bibliographical note

Funding Information:
We thank the Laurentian Educational Center, MN, for permission to collect samples. This research was supported by internal funds from the University of Minnesota.

Publisher Copyright:
© 2016 IUMS.

Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.

Keywords

  • Fatty Acid Methyl Ester
  • Mycobacterium
  • Sarracenia purpurea

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