Mutations that impair a posttranscriptional step in expression of HLA-A and -B antigens

R. DeMars, R. Rudersdorf, C. Chang, J. Petersen, J. Strandtmann, N. Korn, B. Sidwell, H. T. Orr

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Abstract

Mutations can interfere with posttranscriptional expression of the HLA-A and -B genes. B-lymphoblastoid cells that contain one copy of the major histocompatibility complex (MHC) were subjected to mutagenesis and immunoselection for MHC antigen-loss mutants. Some mutations partially reduced surface expression of HLA-A and eliminated HLA-B expression concurrently, although the HLA-A and -B genes were present and transcribed. Antigen expression was fully restored in hybrids of these mutants with other B-lymphoblastoid cells. Therefore, normal cell surface expression of the HLA-A and -B antigens on B lymphoblasts requires (i) execution of at least one trans-active step in the production of the antigens after transcription of the HLA-A and -B genes or (ii) association of the class I antigens with other molecules. DNA analysis of one mutant suggests the possibility that a locus required for the normal expression of the HLA-A and -B antigens is located between the MHC complement genes and the HLA-DP αII locus.

Original languageEnglish (US)
Pages (from-to)8183-8187
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume82
Issue number23
DOIs
StatePublished - Dec 1 1985

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HLA-A Antigens
HLA-B Antigens
Mutation
Major Histocompatibility Complex
Genes
HLA-DP Antigens
Antigens
Histocompatibility Antigens Class I
Histocompatibility Antigens
Mutagenesis
DNA

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Mutations that impair a posttranscriptional step in expression of HLA-A and -B antigens. / DeMars, R.; Rudersdorf, R.; Chang, C.; Petersen, J.; Strandtmann, J.; Korn, N.; Sidwell, B.; Orr, H. T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, No. 23, 01.12.1985, p. 8183-8187.

Research output: Contribution to journalArticle

DeMars, R. ; Rudersdorf, R. ; Chang, C. ; Petersen, J. ; Strandtmann, J. ; Korn, N. ; Sidwell, B. ; Orr, H. T. / Mutations that impair a posttranscriptional step in expression of HLA-A and -B antigens. In: Proceedings of the National Academy of Sciences of the United States of America. 1985 ; Vol. 82, No. 23. pp. 8183-8187.
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AU - Chang, C.

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AU - Korn, N.

AU - Sidwell, B.

AU - Orr, H. T.

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