Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis

Timothy J. Heisel, Chunyao Li, Katia M. Grey, Sue Gibson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Nutrient response networks are likely to have been among the first response networks to evolve, as the ability to sense and respond to the levels of available nutrients is critical for all organisms. Although several forward genetic screens have been successful in identifying components of plant sugar-response networks, many components remain to be identified. Toward this end, a reverse genetic screen was conducted in Arabidopsis thaliana to identify additional components of sugar-response networks. This screen was based on the rationale that some of the genes involved in sugar-response networks are likely to be themselves sugar regulated at the steady-state mRNA level and to encode proteins with activities commonly associated with response networks. This rationale was validated by the identification of hac1 mutants that are defective in sugar response. HAC1 encodes a histone acetyltransferase. Histone acetyltransferases increase transcription of specific genes by acetylating histones associated with those genes. Mutations in HAC1 also cause reduced fertility, a moderate degree of resistance to paclobutrazol and altered transcript levels of specific genes. Previous research has shown that hac1 mutants exhibit delayed flowering. The sugar-response and fertility defects of hac1 mutants may be partially explained by decreased expression of AtPV42a and AtPV42b, which are putative components of plant SnRK1 complexes. SnRK1 complexes have been shown to function as central regulators of plant nutrient and energy status. Involvement of a histone acetyltransferase in sugar response provides a possible mechanism whereby nutritional status could exert long-term effects on plant development and metabolism.

Original languageEnglish (US)
Article number245
JournalFrontiers in Plant Science
Volume4
Issue numberJUL
DOIs
StatePublished - Jul 17 2013

Fingerprint

Arabidopsis
sugars
mutation
gene expression
mutants
nutrients
genes
paclobutrazol
histones
nutritional status
plant development
long term effects
Arabidopsis thaliana
transcription (genetics)
flowering
metabolism
organisms
energy
histone acetyltransferase
proteins

Keywords

  • Arabidopsis
  • Chromatin modification
  • Fertility
  • Histone acetyltransferase
  • SnRK1
  • Sucrose response
  • Sugar response
  • Sugar signaling

Cite this

Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis. / Heisel, Timothy J.; Li, Chunyao; Grey, Katia M.; Gibson, Sue.

In: Frontiers in Plant Science, Vol. 4, No. JUL, 245, 17.07.2013.

Research output: Contribution to journalArticle

@article{bd1ee9eb384e4563983e7274032ceb5e,
title = "Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis",
abstract = "Nutrient response networks are likely to have been among the first response networks to evolve, as the ability to sense and respond to the levels of available nutrients is critical for all organisms. Although several forward genetic screens have been successful in identifying components of plant sugar-response networks, many components remain to be identified. Toward this end, a reverse genetic screen was conducted in Arabidopsis thaliana to identify additional components of sugar-response networks. This screen was based on the rationale that some of the genes involved in sugar-response networks are likely to be themselves sugar regulated at the steady-state mRNA level and to encode proteins with activities commonly associated with response networks. This rationale was validated by the identification of hac1 mutants that are defective in sugar response. HAC1 encodes a histone acetyltransferase. Histone acetyltransferases increase transcription of specific genes by acetylating histones associated with those genes. Mutations in HAC1 also cause reduced fertility, a moderate degree of resistance to paclobutrazol and altered transcript levels of specific genes. Previous research has shown that hac1 mutants exhibit delayed flowering. The sugar-response and fertility defects of hac1 mutants may be partially explained by decreased expression of AtPV42a and AtPV42b, which are putative components of plant SnRK1 complexes. SnRK1 complexes have been shown to function as central regulators of plant nutrient and energy status. Involvement of a histone acetyltransferase in sugar response provides a possible mechanism whereby nutritional status could exert long-term effects on plant development and metabolism.",
keywords = "Arabidopsis, Chromatin modification, Fertility, Histone acetyltransferase, SnRK1, Sucrose response, Sugar response, Sugar signaling",
author = "Heisel, {Timothy J.} and Chunyao Li and Grey, {Katia M.} and Sue Gibson",
year = "2013",
month = "7",
day = "17",
doi = "10.3389/fpls.2013.00245",
language = "English (US)",
volume = "4",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media S. A.",
number = "JUL",

}

TY - JOUR

T1 - Mutations in HISTONE ACETYLTRANSFERASE1 affect sugar response and gene expression in Arabidopsis

AU - Heisel, Timothy J.

AU - Li, Chunyao

AU - Grey, Katia M.

AU - Gibson, Sue

PY - 2013/7/17

Y1 - 2013/7/17

N2 - Nutrient response networks are likely to have been among the first response networks to evolve, as the ability to sense and respond to the levels of available nutrients is critical for all organisms. Although several forward genetic screens have been successful in identifying components of plant sugar-response networks, many components remain to be identified. Toward this end, a reverse genetic screen was conducted in Arabidopsis thaliana to identify additional components of sugar-response networks. This screen was based on the rationale that some of the genes involved in sugar-response networks are likely to be themselves sugar regulated at the steady-state mRNA level and to encode proteins with activities commonly associated with response networks. This rationale was validated by the identification of hac1 mutants that are defective in sugar response. HAC1 encodes a histone acetyltransferase. Histone acetyltransferases increase transcription of specific genes by acetylating histones associated with those genes. Mutations in HAC1 also cause reduced fertility, a moderate degree of resistance to paclobutrazol and altered transcript levels of specific genes. Previous research has shown that hac1 mutants exhibit delayed flowering. The sugar-response and fertility defects of hac1 mutants may be partially explained by decreased expression of AtPV42a and AtPV42b, which are putative components of plant SnRK1 complexes. SnRK1 complexes have been shown to function as central regulators of plant nutrient and energy status. Involvement of a histone acetyltransferase in sugar response provides a possible mechanism whereby nutritional status could exert long-term effects on plant development and metabolism.

AB - Nutrient response networks are likely to have been among the first response networks to evolve, as the ability to sense and respond to the levels of available nutrients is critical for all organisms. Although several forward genetic screens have been successful in identifying components of plant sugar-response networks, many components remain to be identified. Toward this end, a reverse genetic screen was conducted in Arabidopsis thaliana to identify additional components of sugar-response networks. This screen was based on the rationale that some of the genes involved in sugar-response networks are likely to be themselves sugar regulated at the steady-state mRNA level and to encode proteins with activities commonly associated with response networks. This rationale was validated by the identification of hac1 mutants that are defective in sugar response. HAC1 encodes a histone acetyltransferase. Histone acetyltransferases increase transcription of specific genes by acetylating histones associated with those genes. Mutations in HAC1 also cause reduced fertility, a moderate degree of resistance to paclobutrazol and altered transcript levels of specific genes. Previous research has shown that hac1 mutants exhibit delayed flowering. The sugar-response and fertility defects of hac1 mutants may be partially explained by decreased expression of AtPV42a and AtPV42b, which are putative components of plant SnRK1 complexes. SnRK1 complexes have been shown to function as central regulators of plant nutrient and energy status. Involvement of a histone acetyltransferase in sugar response provides a possible mechanism whereby nutritional status could exert long-term effects on plant development and metabolism.

KW - Arabidopsis

KW - Chromatin modification

KW - Fertility

KW - Histone acetyltransferase

KW - SnRK1

KW - Sucrose response

KW - Sugar response

KW - Sugar signaling

UR - http://www.scopus.com/inward/record.url?scp=84896997585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896997585&partnerID=8YFLogxK

U2 - 10.3389/fpls.2013.00245

DO - 10.3389/fpls.2013.00245

M3 - Article

VL - 4

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

IS - JUL

M1 - 245

ER -