The mutagenicity toward S. typhimurium TA 100 and tumor initiating activity on mouse skin of benzo[b]fluoranthene (BbF), 1-methylbenzo[b]fluoranthene (1-MeBbF), 3-MeBbF, 7-MeBbF, 8-MeBbF, 9-MeBbF, 12-MeBbF, 5,6-dimethylbenzo[b]fluoranthene (5,6-diMeBbF) and 1,3-diMeBbF were assayed. Dose-dependent mutagenic activity was observed for BbF, 3-MeBbF, and 1,3-diMeBbF; the other compounds were inactive at the doses tested. 3-MeBbF and 1,3-diMeBbF were strong tumor initiators, with activity greater than that of BbF. All the other compounds were less tumorigenic than BbF. The results suggest that the structural features favoring tumorigenicity of methylated non-alternant polynuclear aromatic hydrocarbons such as BbF are different from those favoring tumorigenicity of methylated alternant polynuclear aromatic hydrocarbons such as benzo[a]pyrene, chrysene and benz[a]anthracene.
Bibliographical noteFunding Information:
A Study of Chemical Carcinogenesis, 81. This study was supported by NCI Grant CA-32242. We thank Lynn Radok for her assistance in the tumorigenicity assays, Aibertina Swanson for carrying out the mutagenicity studies, and Lori DeMarco for preparing the manuscript.