Mutagenesis and High-Throughput Functional Genomics in Cereal Crops: Current Status

H. S. Balyan; N. Sreenivasulu; O. Riera-Lizarazu; P. Azhaguvel; S. F. Kianian

doi:10.1016/S0065-2113(08)00207-1

Mutagenesis and High-Throughput Functional Genomics in Cereal Crops: Current Status

H. S. Balyan
, N. Sreenivasulu
, O. Riera-Lizarazu
, P. Azhaguvel
, S. F. Kianian

Research output: Chapter in Book/Report/Conference proceeding › Chapter

7 Scopus citations

Abstract

The accumulation of large sequence information in cereal crop plants has opened opportunities for determining gene function using reverse genetics rather than forward-genetics approaches. A number of reverse-genetics approaches have been developed and used in model plant species such as Arabidopsis and rice and recently been extended to other cereal crop species during the last few years to determine gene-function relationship. Insertional and chemical/physical mutagen induced mutagenesis combined with emerging high-throughput analytical approaches have made it possible to rapidly discover sequence-function relationship through reverse genetics at genome-wide scale, even in large genome cereal species. In this review, we provide a brief glimpse of the insertional mutagenesis (T-DNA and transposons) based approaches and thereafter discuss the recently discovered non-transgenic technologies such as TILLING (Targeting Induced Local Lesions IN Genomes) and DEALING (Detecting Adduct Lesions In Genomes), and their emerging applications in cereal crop plants using suitable examples.

Original language	English (US)
Title of host publication	Advances in Agronomy
Editors	Donald Sparks
Pages	357-414
Number of pages	58
DOIs	https://doi.org/10.1016/S0065-2113(08)00207-1
State	Published - 2008
Externally published	Yes

Publication series

Name	Advances in Agronomy
Volume	98
ISSN (Print)	0065-2113

Bibliographical note

Funding Information:
The exchange of scientists award by INSA‐DFG and the Developing Country Collaboration award by the NSF, USA, to HSB are gratefully acknowledged, during the tenure of which this chapter was conceived and prepared. The DEALING research by ORL and SFK was supported by a grant from the National Science Foundation‐Plant Genome Research Program Contract Agreement No. DBI‐0321462.

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abstract = "The accumulation of large sequence information in cereal crop plants has opened opportunities for determining gene function using reverse genetics rather than forward-genetics approaches. A number of reverse-genetics approaches have been developed and used in model plant species such as Arabidopsis and rice and recently been extended to other cereal crop species during the last few years to determine gene-function relationship. Insertional and chemical/physical mutagen induced mutagenesis combined with emerging high-throughput analytical approaches have made it possible to rapidly discover sequence-function relationship through reverse genetics at genome-wide scale, even in large genome cereal species. In this review, we provide a brief glimpse of the insertional mutagenesis (T-DNA and transposons) based approaches and thereafter discuss the recently discovered non-transgenic technologies such as TILLING (Targeting Induced Local Lesions IN Genomes) and DEALING (Detecting Adduct Lesions In Genomes), and their emerging applications in cereal crop plants using suitable examples.",

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note = "Funding Information: The exchange of scientists award by INSA‐DFG and the Developing Country Collaboration award by the NSF, USA, to HSB are gratefully acknowledged, during the tenure of which this chapter was conceived and prepared. The DEALING research by ORL and SFK was supported by a grant from the National Science Foundation‐Plant Genome Research Program Contract Agreement No. DBI‐0321462. ",

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doi = "10.1016/S0065-2113(08)00207-1",

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AU - Balyan, H. S.

AU - Sreenivasulu, N.

AU - Riera-Lizarazu, O.

AU - Azhaguvel, P.

AU - Kianian, S. F.

N1 - Funding Information: The exchange of scientists award by INSA‐DFG and the Developing Country Collaboration award by the NSF, USA, to HSB are gratefully acknowledged, during the tenure of which this chapter was conceived and prepared. The DEALING research by ORL and SFK was supported by a grant from the National Science Foundation‐Plant Genome Research Program Contract Agreement No. DBI‐0321462.

PY - 2008

Y1 - 2008

N2 - The accumulation of large sequence information in cereal crop plants has opened opportunities for determining gene function using reverse genetics rather than forward-genetics approaches. A number of reverse-genetics approaches have been developed and used in model plant species such as Arabidopsis and rice and recently been extended to other cereal crop species during the last few years to determine gene-function relationship. Insertional and chemical/physical mutagen induced mutagenesis combined with emerging high-throughput analytical approaches have made it possible to rapidly discover sequence-function relationship through reverse genetics at genome-wide scale, even in large genome cereal species. In this review, we provide a brief glimpse of the insertional mutagenesis (T-DNA and transposons) based approaches and thereafter discuss the recently discovered non-transgenic technologies such as TILLING (Targeting Induced Local Lesions IN Genomes) and DEALING (Detecting Adduct Lesions In Genomes), and their emerging applications in cereal crop plants using suitable examples.

AB - The accumulation of large sequence information in cereal crop plants has opened opportunities for determining gene function using reverse genetics rather than forward-genetics approaches. A number of reverse-genetics approaches have been developed and used in model plant species such as Arabidopsis and rice and recently been extended to other cereal crop species during the last few years to determine gene-function relationship. Insertional and chemical/physical mutagen induced mutagenesis combined with emerging high-throughput analytical approaches have made it possible to rapidly discover sequence-function relationship through reverse genetics at genome-wide scale, even in large genome cereal species. In this review, we provide a brief glimpse of the insertional mutagenesis (T-DNA and transposons) based approaches and thereafter discuss the recently discovered non-transgenic technologies such as TILLING (Targeting Induced Local Lesions IN Genomes) and DEALING (Detecting Adduct Lesions In Genomes), and their emerging applications in cereal crop plants using suitable examples.

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M3 - Chapter

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SN - 9780123743558

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BT - Advances in Agronomy

A2 - Sparks, Donald

ER -

Mutagenesis and High-Throughput Functional Genomics in Cereal Crops: Current Status

Abstract

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Bibliographical note

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