Muscle proteolysis and weight loss in a neonatal rat model of sepsis syndrome

D. M. Premer, R. Goertz, Michael K Georgieff, Mark C Mammel, Sarah J Schwarzenberg

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Our hypothesis is that nitrogen loss in septic neonates is caused by increased muscle proteolysis. Sprague-Dawley rat pups (P7) were injected intraperitoneally with NaCl or 4 mg/kg/BW lipopolysaccharide (LPS) and then sacrificed at 2, 4, 24, and 48 hr. Sepsis syndrome was confirmed by elevated serum tumor necrosis factor (24.6 ng/mL ± 18.4 [LPS] and <1.0 ng/mL [controls]; p < .05). Proteolysis in gastrocnemius/soleus muscle was analyzed by quantitation of tissue tyrosine loss. The neonatal rats injected with LPS had significant media tyrosine release at 24 hr compared to the controls (0.39 ± 0.14 versus 0.25 ± 0.11 μmol tyrosine/g muscle; p < .05). At 48 hr, LPS-induced muscle tyrosine release ceased (0.24 ± 0.04 [control] versus 0.23 ± 0.03 μmol tyrosine/g muscle [LPS]). After 48 hr, gastrocnemium/soleus weight was less in the LPS-injected rats (50.5 ± 4.8 to 31.2 ± 4.0 g; p < .0001). Similar changes were not seen in the extensor digitorum longus, suggesting that some muscles were relatively preserved. Also, LPS resulted in significant weight loss. We conclude that selective muscle proteolysis contributes to nitrogen loss in neonatal sepsis. Although proteolysis abates by 48 hr, short-term injury results in significant muscle-mass deficit.

Original languageEnglish (US)
Pages (from-to)97-101
Number of pages5
JournalInflammation
Volume26
Issue number2
DOIs
StatePublished - Apr 15 2002

Fingerprint

Systemic Inflammatory Response Syndrome
Proteolysis
Lipopolysaccharides
Weight Loss
Muscles
Tyrosine
Skeletal Muscle
Nitrogen
Sprague Dawley Rats
Tumor Necrosis Factor-alpha
Weights and Measures
Wounds and Injuries
Serum

Keywords

  • Muscle
  • Neonate
  • Protein
  • Sepsis

Cite this

Muscle proteolysis and weight loss in a neonatal rat model of sepsis syndrome. / Premer, D. M.; Goertz, R.; Georgieff, Michael K; Mammel, Mark C; Schwarzenberg, Sarah J.

In: Inflammation, Vol. 26, No. 2, 15.04.2002, p. 97-101.

Research output: Contribution to journalArticle

@article{91d6eb3e101c4130a89573142eacb83e,
title = "Muscle proteolysis and weight loss in a neonatal rat model of sepsis syndrome",
abstract = "Our hypothesis is that nitrogen loss in septic neonates is caused by increased muscle proteolysis. Sprague-Dawley rat pups (P7) were injected intraperitoneally with NaCl or 4 mg/kg/BW lipopolysaccharide (LPS) and then sacrificed at 2, 4, 24, and 48 hr. Sepsis syndrome was confirmed by elevated serum tumor necrosis factor (24.6 ng/mL ± 18.4 [LPS] and <1.0 ng/mL [controls]; p < .05). Proteolysis in gastrocnemius/soleus muscle was analyzed by quantitation of tissue tyrosine loss. The neonatal rats injected with LPS had significant media tyrosine release at 24 hr compared to the controls (0.39 ± 0.14 versus 0.25 ± 0.11 μmol tyrosine/g muscle; p < .05). At 48 hr, LPS-induced muscle tyrosine release ceased (0.24 ± 0.04 [control] versus 0.23 ± 0.03 μmol tyrosine/g muscle [LPS]). After 48 hr, gastrocnemium/soleus weight was less in the LPS-injected rats (50.5 ± 4.8 to 31.2 ± 4.0 g; p < .0001). Similar changes were not seen in the extensor digitorum longus, suggesting that some muscles were relatively preserved. Also, LPS resulted in significant weight loss. We conclude that selective muscle proteolysis contributes to nitrogen loss in neonatal sepsis. Although proteolysis abates by 48 hr, short-term injury results in significant muscle-mass deficit.",
keywords = "Muscle, Neonate, Protein, Sepsis",
author = "Premer, {D. M.} and R. Goertz and Georgieff, {Michael K} and Mammel, {Mark C} and Schwarzenberg, {Sarah J}",
year = "2002",
month = "4",
day = "15",
doi = "10.1023/A:1014840412552",
language = "English (US)",
volume = "26",
pages = "97--101",
journal = "Inflammation",
issn = "0360-3997",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - Muscle proteolysis and weight loss in a neonatal rat model of sepsis syndrome

AU - Premer, D. M.

AU - Goertz, R.

AU - Georgieff, Michael K

AU - Mammel, Mark C

AU - Schwarzenberg, Sarah J

PY - 2002/4/15

Y1 - 2002/4/15

N2 - Our hypothesis is that nitrogen loss in septic neonates is caused by increased muscle proteolysis. Sprague-Dawley rat pups (P7) were injected intraperitoneally with NaCl or 4 mg/kg/BW lipopolysaccharide (LPS) and then sacrificed at 2, 4, 24, and 48 hr. Sepsis syndrome was confirmed by elevated serum tumor necrosis factor (24.6 ng/mL ± 18.4 [LPS] and <1.0 ng/mL [controls]; p < .05). Proteolysis in gastrocnemius/soleus muscle was analyzed by quantitation of tissue tyrosine loss. The neonatal rats injected with LPS had significant media tyrosine release at 24 hr compared to the controls (0.39 ± 0.14 versus 0.25 ± 0.11 μmol tyrosine/g muscle; p < .05). At 48 hr, LPS-induced muscle tyrosine release ceased (0.24 ± 0.04 [control] versus 0.23 ± 0.03 μmol tyrosine/g muscle [LPS]). After 48 hr, gastrocnemium/soleus weight was less in the LPS-injected rats (50.5 ± 4.8 to 31.2 ± 4.0 g; p < .0001). Similar changes were not seen in the extensor digitorum longus, suggesting that some muscles were relatively preserved. Also, LPS resulted in significant weight loss. We conclude that selective muscle proteolysis contributes to nitrogen loss in neonatal sepsis. Although proteolysis abates by 48 hr, short-term injury results in significant muscle-mass deficit.

AB - Our hypothesis is that nitrogen loss in septic neonates is caused by increased muscle proteolysis. Sprague-Dawley rat pups (P7) were injected intraperitoneally with NaCl or 4 mg/kg/BW lipopolysaccharide (LPS) and then sacrificed at 2, 4, 24, and 48 hr. Sepsis syndrome was confirmed by elevated serum tumor necrosis factor (24.6 ng/mL ± 18.4 [LPS] and <1.0 ng/mL [controls]; p < .05). Proteolysis in gastrocnemius/soleus muscle was analyzed by quantitation of tissue tyrosine loss. The neonatal rats injected with LPS had significant media tyrosine release at 24 hr compared to the controls (0.39 ± 0.14 versus 0.25 ± 0.11 μmol tyrosine/g muscle; p < .05). At 48 hr, LPS-induced muscle tyrosine release ceased (0.24 ± 0.04 [control] versus 0.23 ± 0.03 μmol tyrosine/g muscle [LPS]). After 48 hr, gastrocnemium/soleus weight was less in the LPS-injected rats (50.5 ± 4.8 to 31.2 ± 4.0 g; p < .0001). Similar changes were not seen in the extensor digitorum longus, suggesting that some muscles were relatively preserved. Also, LPS resulted in significant weight loss. We conclude that selective muscle proteolysis contributes to nitrogen loss in neonatal sepsis. Although proteolysis abates by 48 hr, short-term injury results in significant muscle-mass deficit.

KW - Muscle

KW - Neonate

KW - Protein

KW - Sepsis

UR - http://www.scopus.com/inward/record.url?scp=0036220486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036220486&partnerID=8YFLogxK

U2 - 10.1023/A:1014840412552

DO - 10.1023/A:1014840412552

M3 - Article

C2 - 11989793

AN - SCOPUS:0036220486

VL - 26

SP - 97

EP - 101

JO - Inflammation

JF - Inflammation

SN - 0360-3997

IS - 2

ER -