Jose Barbosa, Blanche M Chavers, Michael W Steffes, Edward Szalapski, Richard A. Cohen, Alfred F. Michael, Byron Hoogwerf, Michael Mauer

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17 Scopus citations


Markers for prediabetes were sought in a study of 29 non-diabetic sibs of patients with insulin-dependent diabetes, 18 of whom were HLA-identical, and 11 non-HLA identical, to their diabetic sibs, and 47 controls. HLA-identical sibs had higher mean plasma glucose levels than controls at 60, 90, and 120 min during oral glucose tolerance tests (GTT). Similarly, mean serum insulin levels were higher in HLA-identical sibs than in controls at 60 and 90 min during the GTT. Non-HLA-identical sibs had higher glucose and insulin levels only at 60 min when compared to controls, but they were not different from HLA-identical sibs at any time during the GTT. HLA-identical sibs had significantly more intense immunofluorescent (IF) staining of albumin in the skeletal muscle extracellular membrane than either non-HLA-identical sibs or controls. IF staining for IgG in the skeletal muscle extracellular membrane was significantly more intense in both sib groups than in the controls. Muscle capillary basement thickness was normal in both sib groups. There were no significant differences among the three groups for glycosylated hæmoglobin and cytoplasmic islet-cell antibodies. Since HLA identity with the proband is likely to be a marker for prediabetes in diabetic families, the abnormal IF staining of muscle extracellular membrane for protein is likely to be an expression of the prediabetic state and may be related to mild, possibly intermittent, glucose intolerance.

Original languageEnglish (US)
Pages (from-to)330-333
Number of pages4
JournalThe Lancet
Issue number8190
StatePublished - 1980

Bibliographical note

Funding Information:
The research was supported by grants AM24171 and HL22479 from the National Institutes of Health and CRC grant RR00833, and by grants-in-aid from the American Heart Association (California affiliate) and the Cooley’s Anemia Foundation of New York. J. L. was supported by a Scripps institu- tional research fellowship. We thank Dr E. Beutler, Dr H. Carloss, Dr D. Cowan, Dr W. H. Crosby, Dr R. McMillan, Dr E. Rosenbaum, and Dr D. Schwartz for allowing us to study patients under their care. We also thank Shirley Hosier, R.N. and the nursing staff of the General Clinical Research Centre of Scripps Clinic and Research Foundation for their assistance during these studies.


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