Muscle-based gene therapy and tissue engineering for treatment of growth plate injuries

Chang Woo Lee, Vladimir Martinek, Arvydas Usas, Doug Musgrave, E. A. Pickvance, Paul Robbins, Morey S. Moreland, Freddie H. Fu, Johnny Huard

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Growth plate injuries may lead to a progressive angular deformity or longitudinal growth disturbance. The authors investigated the feasibility of gene therapy and tissue engineering based on autologous muscle- and adenoviral-mediated gene transfer of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-2 (BMP-2) to treat tibial physeal defects in rabbits. The medial half of the left proximal tibial growth plate was completely excised in 44 6-week-old New Zealand white rabbits. Four experimental groups were created: no treatment (I), autologous muscle interposition (II), autologous muscle interposition injected with adIGF-1 (III), and autologous muscle interposition injected with adBMP-2 (IV). Radiographic and histologic assessments were obtained postoperatively. Significant tibial shortening and a compact osseous bridge were observed in groups I and IV. Growth plates remained open in groups II and III. This experiment demonstrates that IGF-1 had a supportive effect on physeal chondrocytes, while BMP-2 caused increased osteogenic activity in the injured growth plates.

Original languageEnglish (US)
Pages (from-to)565-572
Number of pages8
JournalJournal of Pediatric Orthopaedics
Issue number5
StatePublished - 2002
Externally publishedYes


  • BMP-2
  • Gene therapy
  • IGF-1
  • Muscle
  • Physeal defect
  • Tissue engineering


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