TY - JOUR
T1 - Murine cell surface immunoglobulin
T2 - Two forms of δ-heavy chain
AU - Mescher, M. F.
AU - Pollock, R. R.
PY - 1979
Y1 - 1979
N2 - The heavy chain of isolated murine cell surface IgD is present in two forms, separable by electrophoresis on SDS-polyacrylamide gradient gels. Both forms of δ-heavy chain are present on the surface of intact spleen cells and have apparent m.w. of approximately 70,000 (δ1) and 68,000 (δ2). Treatment of surface IgD with neuraminidase before isolation results in a single IgD heavy chain band on SDS gels having an apparent m.w. of 65,000, indicating that δ1 and δ2 differ in sialic acid content. Δ2 is removed from the cell surface by papain more readily than δ1, suggesting a possible functional significance for the two forms.
AB - The heavy chain of isolated murine cell surface IgD is present in two forms, separable by electrophoresis on SDS-polyacrylamide gradient gels. Both forms of δ-heavy chain are present on the surface of intact spleen cells and have apparent m.w. of approximately 70,000 (δ1) and 68,000 (δ2). Treatment of surface IgD with neuraminidase before isolation results in a single IgD heavy chain band on SDS gels having an apparent m.w. of 65,000, indicating that δ1 and δ2 differ in sialic acid content. Δ2 is removed from the cell surface by papain more readily than δ1, suggesting a possible functional significance for the two forms.
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M3 - Article
C2 - 112186
AN - SCOPUS:0018292326
SN - 0022-1767
VL - 123
SP - 1155
EP - 1161
JO - Journal of Immunology
JF - Journal of Immunology
IS - 3
ER -