Murihexocin C from the leaves of Annona squamosa Linn. Induces apoptosis in human colon carcinoma Col 2 cell line

Ahmad Reza F. Mazahery, Romel P. Dator, Gisela P. Concepcion, Sonia D. Jacinto

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The acetogenin murihexocin C was isolated from the leaves of Annona squamosa Linn. The structure was assigned based on 1H and 13C NMR and HR-ESI-TOF-MS. This is the first report of the compound from this plant. Murihexocin C exhibited significant cytotoxicity to human colon carcinoma Col 2 cells (IC 50 0.50 μg mL -1 or 0.79 μM) and normal Chinese hamster ovarian cells AA8 cells (IC 50 0.78 μg mL -1 or 1.24 μM). Murihexocin C (50 μg mL -1) induced extensive apoptotic cell death in Col 2 cells at 12 h as demonstrated by the TUNEL assay. Activation of the apoptotic effector caspase 3 was observed and a Bcl-2-like protein was noted to be down regulated.

Original languageEnglish (US)
Pages (from-to)122-132
Number of pages11
JournalPhilippine Agricultural Scientist
Volume92
Issue number2
StatePublished - Jun 1 2009
Externally publishedYes

Keywords

  • Anticancer
  • Apoptosis
  • Cell culture
  • Chromatography
  • Colon cancer
  • Cytotoxicity
  • Natural products

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