Multivalent glycocluster design through directed evolution

Iain S. MacPherson, J. Sebastian Temme, Sevan Habeshian, Krzysztof Felczak, Krzysztof Pankiewicz, Lizbeth Hedstrom, Isaac J. Krauss

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Selection with modified aptamers: A method is described for design of glycocluster ligands by directed evolution. Glycan azides are attached to a library of alkyne-containing DNA sequences. DNA sequences which cluster the glycans best for multivalent binding to the target are selected and amplified. This method has been applied to the development of high-mannose clusters which bind HIV-neutralizing antibody 2G12.

Original languageEnglish (US)
Pages (from-to)11238-11242
Number of pages5
JournalAngewandte Chemie - International Edition
Volume50
Issue number47
DOIs
StatePublished - Nov 18 2011

Keywords

  • carbohydrates
  • cluster effect
  • directed evolution
  • glycoconjugates
  • multivalency

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