Selection with modified aptamers: A method is described for design of glycocluster ligands by directed evolution. Glycan azides are attached to a library of alkyne-containing DNA sequences. DNA sequences which cluster the glycans best for multivalent binding to the target are selected and amplified. This method has been applied to the development of high-mannose clusters which bind HIV-neutralizing antibody 2G12.
|Original language||English (US)|
|Number of pages||5|
|Journal||Angewandte Chemie - International Edition|
|State||Published - Nov 18 2011|
- cluster effect
- directed evolution