Multispectral phloem-mobile probes: Properties and applications

Michael Knoblauch, Marc Vendrell, Erica De Leau, Andrea Paterlini, Kirsten Knox, Tim Ross-Elliot, Anke Reinders, Stephen A. Brockman, John Ward, Karl Oparka

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Using Arabidopsis (Arabidopsis thaliana) seedlings, we identified a range of small fluorescent probes that entered the translocation stream and were unloaded at the root tip. These probes had absorbance/emission maxima ranging from 367/454 to 546/576 nm and represent a versatile toolbox for studying phloem transport. Of the probes that we tested, naturally occurring fluorescent coumarin glucosides (esculin and fraxin) were phloem loaded and transported in oocytes by the sucrose transporter, AtSUC2. Arabidopsis plants in which AtSUC2 was replaced with barley (Hordeum vulgare) sucrose transporter (HvSUT1), which does not transport esculin in oocytes, failed to load esculin into the phloem. In wild-type plants, the fluorescence of esculin decayed to background levels about 2 h after phloem unloading, making it a suitable tracer for pulse-labeling studies of phloem transport. We identified additional probes, such as carboxytetraethylrhodamine, a red fluorescent probe that, unlike esculin, was stable for several hours after phloem unloading and could be used to study phloem transport in Arabidopsis lines expressing green fluorescent protein.

Original languageEnglish (US)
Pages (from-to)1211-1220
Number of pages10
JournalPlant physiology
Volume167
Issue number4
DOIs
StatePublished - Apr 1 2015

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Phloem
Esculin
phloem
Arabidopsis
Hordeum
Fluorescent Dyes
Oocytes
Sucrose
transporters
oocytes
sucrose
Meristem
coumarin
Glucosides
Green Fluorescent Proteins
Seedlings
green fluorescent protein
root tips
Hordeum vulgare
absorbance

Cite this

Knoblauch, M., Vendrell, M., De Leau, E., Paterlini, A., Knox, K., Ross-Elliot, T., ... Oparka, K. (2015). Multispectral phloem-mobile probes: Properties and applications. Plant physiology, 167(4), 1211-1220. https://doi.org/10.1104/pp.114.255414

Multispectral phloem-mobile probes : Properties and applications. / Knoblauch, Michael; Vendrell, Marc; De Leau, Erica; Paterlini, Andrea; Knox, Kirsten; Ross-Elliot, Tim; Reinders, Anke; Brockman, Stephen A.; Ward, John; Oparka, Karl.

In: Plant physiology, Vol. 167, No. 4, 01.04.2015, p. 1211-1220.

Research output: Contribution to journalArticle

Knoblauch, M, Vendrell, M, De Leau, E, Paterlini, A, Knox, K, Ross-Elliot, T, Reinders, A, Brockman, SA, Ward, J & Oparka, K 2015, 'Multispectral phloem-mobile probes: Properties and applications', Plant physiology, vol. 167, no. 4, pp. 1211-1220. https://doi.org/10.1104/pp.114.255414
Knoblauch M, Vendrell M, De Leau E, Paterlini A, Knox K, Ross-Elliot T et al. Multispectral phloem-mobile probes: Properties and applications. Plant physiology. 2015 Apr 1;167(4):1211-1220. https://doi.org/10.1104/pp.114.255414
Knoblauch, Michael ; Vendrell, Marc ; De Leau, Erica ; Paterlini, Andrea ; Knox, Kirsten ; Ross-Elliot, Tim ; Reinders, Anke ; Brockman, Stephen A. ; Ward, John ; Oparka, Karl. / Multispectral phloem-mobile probes : Properties and applications. In: Plant physiology. 2015 ; Vol. 167, No. 4. pp. 1211-1220.
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