Multisite study of cryptosporidiosis in children with diarrhea in India

Sitara Swarna Rao Ajjampur, Farzana Begum Liakath, Arun Kannan, Priya Rajendran, Rajiv Sarkar, Prabhakar Devarajan Moses, Anna Simon, Indira Agarwal, Ann Mathew, Roberta O'Connor, Honorine Ward, Gagandeep Kang

Research output: Contribution to journalArticlepeer-review

64 Scopus citations

Abstract

Cryptosporidium spp., a common cause of diarrhea in children, were investigated in the first multisite study in India. Diarrheal stools from hospitalized children aged <5 years from Delhi, Trichy, and Vellore were analyzed by microscopy, PCR-restriction fragment length polymorphism (RFLP), and/or sequencing at the small-subunit (SSU) rRNA and Cpgp40/15 loci for species determination and subgenotyping, respectively. Seventy of 2,579 (2.7%) children, 75% of whom were <2 years old, had cryptosporidial diarrhea as determined by microscopy. Genotyping and subgenotyping showed that Cryptosporidium hominis was the most commonly identified species (59/67 children), and subgenotypes Ie, Ia, Ib, and Id were common in all centers. A novel C. parvum subgenotype, IIn, was identified in Vellore. Meteorological analysis revealed a higher rate of cryptosporidial positivity during hotter and drier weather in Delhi.

Original languageEnglish (US)
Pages (from-to)2075-2081
Number of pages7
JournalJournal of clinical microbiology
Volume48
Issue number6
DOIs
StatePublished - Jun 2010
Externally publishedYes

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