TY - JOUR
T1 - Multisite study of cryptosporidiosis in children with diarrhea in India
AU - Ajjampur, Sitara Swarna Rao
AU - Liakath, Farzana Begum
AU - Kannan, Arun
AU - Rajendran, Priya
AU - Sarkar, Rajiv
AU - Moses, Prabhakar Devarajan
AU - Simon, Anna
AU - Agarwal, Indira
AU - Mathew, Ann
AU - O'Connor, Roberta
AU - Ward, Honorine
AU - Kang, Gagandeep
PY - 2010/6
Y1 - 2010/6
N2 - Cryptosporidium spp., a common cause of diarrhea in children, were investigated in the first multisite study in India. Diarrheal stools from hospitalized children aged <5 years from Delhi, Trichy, and Vellore were analyzed by microscopy, PCR-restriction fragment length polymorphism (RFLP), and/or sequencing at the small-subunit (SSU) rRNA and Cpgp40/15 loci for species determination and subgenotyping, respectively. Seventy of 2,579 (2.7%) children, 75% of whom were <2 years old, had cryptosporidial diarrhea as determined by microscopy. Genotyping and subgenotyping showed that Cryptosporidium hominis was the most commonly identified species (59/67 children), and subgenotypes Ie, Ia, Ib, and Id were common in all centers. A novel C. parvum subgenotype, IIn, was identified in Vellore. Meteorological analysis revealed a higher rate of cryptosporidial positivity during hotter and drier weather in Delhi.
AB - Cryptosporidium spp., a common cause of diarrhea in children, were investigated in the first multisite study in India. Diarrheal stools from hospitalized children aged <5 years from Delhi, Trichy, and Vellore were analyzed by microscopy, PCR-restriction fragment length polymorphism (RFLP), and/or sequencing at the small-subunit (SSU) rRNA and Cpgp40/15 loci for species determination and subgenotyping, respectively. Seventy of 2,579 (2.7%) children, 75% of whom were <2 years old, had cryptosporidial diarrhea as determined by microscopy. Genotyping and subgenotyping showed that Cryptosporidium hominis was the most commonly identified species (59/67 children), and subgenotypes Ie, Ia, Ib, and Id were common in all centers. A novel C. parvum subgenotype, IIn, was identified in Vellore. Meteorological analysis revealed a higher rate of cryptosporidial positivity during hotter and drier weather in Delhi.
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U2 - 10.1128/JCM.02509-09
DO - 10.1128/JCM.02509-09
M3 - Article
C2 - 20392919
AN - SCOPUS:77952910568
SN - 0095-1137
VL - 48
SP - 2075
EP - 2081
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 6
ER -