Multiplexed aberration measurement for deep tissue imaging in vivo

Chen Wang; Rui Liu; Daniel E. Milkie; Wenzhi Sun; Zhongchao Tan; Aaron Kerlin; Tsai Wen Chen; Douglas S. Kim; Na Ji

doi:10.1038/nmeth.3068

Multiplexed aberration measurement for deep tissue imaging in vivo

Chen Wang
, Rui Liu
, Daniel E. Milkie
, Wenzhi Sun
, Zhongchao Tan
, Aaron Kerlin
, Tsai Wen Chen
, Douglas S. Kim
, Na Ji

Research output: Contribution to journal › Article › peer-review

131 Scopus citations

Abstract

We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.

Original language	English (US)
Pages (from-to)	1037-1040
Number of pages	4
Journal	Nature Methods
Volume	11
Issue number	10
DOIs	https://doi.org/10.1038/nmeth.3068
State	Published - Jan 1 2014
Externally published	Yes

Bibliographical note

Funding Information:
We thank our colleagues at Janelia Farm Research Campus, Howard Hughes Medical Institute: E. Betzig for helpful discussions; H. Dana, B. MacLennan, G. Ranganathan, K. Smith for help with mice samples; and P. Keller and M. Ahrens for providing zebrafish samples. We thank C. Fang-Yen for advice on C. elegans samples. This work was supported by Howard Hughes Medical Institute.

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AU - Liu, Rui

AU - Milkie, Daniel E.

AU - Sun, Wenzhi

AU - Tan, Zhongchao

AU - Kerlin, Aaron

AU - Chen, Tsai Wen

AU - Kim, Douglas S.

AU - Ji, Na

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Multiplexed aberration measurement for deep tissue imaging in vivo

Abstract

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