Multiplexed aberration measurement for deep tissue imaging in vivo

Chen Wang, Rui Liu, Daniel E. Milkie, Wenzhi Sun, Zhongchao Tan, Aaron Kerlin, Tsai Wen Chen, Douglas S. Kim, Na Ji

Research output: Contribution to journalArticlepeer-review

109 Scopus citations


We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.

Original languageEnglish (US)
Pages (from-to)1037-1040
Number of pages4
JournalNature Methods
Issue number10
StatePublished - Jan 1 2014
Externally publishedYes

Bibliographical note

Funding Information:
We thank our colleagues at Janelia Farm Research Campus, Howard Hughes Medical Institute: E. Betzig for helpful discussions; H. Dana, B. MacLennan, G. Ranganathan, K. Smith for help with mice samples; and P. Keller and M. Ahrens for providing zebrafish samples. We thank C. Fang-Yen for advice on C. elegans samples. This work was supported by Howard Hughes Medical Institute.


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