Multiple roles for MRE11 at uncapped telomeres

Yibin Deng, Xiaolan Guo, David O. Ferguson, Sandy Chang

Research output: Contribution to journalArticlepeer-review

160 Scopus citations

Abstract

Progressive telomere attrition or uncapping of the shelterin complex elicits a DNA damage response as a result of a cell's inability to distinguish dysfunctional telomeric ends from DNA double-strand breaks. Telomere deprotection activates both ataxia telangiectasia mutated (ATM) and telangiectasia and Rad3-related (ATR) kinase-dependent DNA damage response pathways, and promotes efficient non-homologous end-joining (NHEJ) of dysfunctional telomeres. The mammalian MRE11-RAD50-NBS1 (MRN; NBS1 is also known as NBN) complex interacts with ATM to sense chromosomal double-strand breaks and coordinate global DNA damage responses. Although the MRN complex accumulates at dysfunctional telomeres, it is not known whether mammalian MRN promotes repair at these sites. Here we address this question by using mouse alleles that either inactivate the entire MRN complex or eliminate only the nuclease activities of MRE11 (ref. 8). We show that cells lacking MRN do not activate ATM when telomeric repeat binding factor 2 (TRF2) is removed from telomeres, and ligase 4 (LIG4)-dependent chromosome end-to-end fusions are markedly reduced. Residual chromatid fusions involve only telomeres generated by leading strand synthesis. Notably, although cells deficient for MRE11 nuclease activity efficiently activate ATM and recruit 53BP1 (also known as TP53BP1) to deprotected telomeres, the 3′ telomeric overhang persists to prevent NHEJ-mediated chromosomal fusions. Removal of shelterin proteins that protect the 3′ overhang in the setting of MRE11 nuclease deficiency restores LIG4-dependent chromosome fusions. Our data indicate a critical role for the MRN complex in sensing dysfunctional telomeres, and show that in the absence of TRF2, MRE11 nuclease activity removes the 3′ telomeric overhang to promote chromosome fusions. MRE11 can also protect newly replicated leading strand telomeres from NHEJ by promoting 5′ strand resection to generate POT1a-TPP1-bound 3′ overhangs.

Original languageEnglish (US)
Pages (from-to)914-918
Number of pages5
JournalNature
Volume460
Issue number7257
DOIs
StatePublished - Aug 13 2009

Bibliographical note

Funding Information:
Acknowledgements We are grateful to M. Zhao, Z. Lu, P. Iyengar and J. Buis for technical help. Both J. Karlseder and P. Carpenter are thanked for providing antibodies. S.C. acknowledges financial support from the National Institute on Aging (NIA) (RO1 AG028888), the National Cancer Institute (NCI) (RO1 CA129037), the Welch Foundation, the Susan G. Koman Race for the Cure Foundation, the Abraham and Phyllis Katz Foundation and the Michael Kadoorie Cancer Genetic Research Program. Y.D. was supported by an NCI Howard Temin Award (K01CA124461).

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