Multiple dendritic cell populations activate CD4+ T cells after viral stimulation

Adele M. Mount, Christopher M. Smith, Fiona Kupresanin, Kristina Stoermer, William R. Heath, Gabrielle T. Belz

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Dendritic cells (DC) are a heterogeneous cell population that bridge the innate and adaptive immune systems. CD8α DC play a prominent and sometimes exclusive, role in driving amplification of CD8+ T cells during a viral infection. Whether this reliance on a single subset of DC also applies for CD4+ T cell activation is unknown. We used a direct ex vivo antigen presentation assay to probe the capacity of flow cytometrically purified DC populations to drive amplification of CD4+ and CD8+ T cells following infection with influenza virus by different routes. This study examined the contributions of non-CD82a DC populations in the amplification of CD8+ and CD4+ T cells in cutaneous and systemic influenza viral infections. We confirmed that in vivo, effective immune responses for CD8+ T cells are dominated by presentation of antiger by CD8α DC but can involve non-CD8a DC. In contrast, CD4+ T cell responses relied more heavily on the contributions of dermal DC migrating from peripheral lymphoid tissues following cutaneous infection, and CD4 DC in the spleen after systemic infection. CD4+ T cell priming by DC subsets that is dependent upon the route of administration raises the possibility that vaccination approaches could be tailored to prime helper T cell immunity. Copyright:

Original languageEnglish (US)
Article numbere1691
JournalPloS one
Volume3
Issue number2
DOIs
StatePublished - Feb 27 2008

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