Multiphoton fluorescence lifetime imaging microscopy (FLIM) using a streak camera

R. V. Krishnan; H. Saitoh; H. Terada; V. E. Centonze; B. Herman

doi:10.1117/12.478001

Multiphoton fluorescence lifetime imaging microscopy (FLIM) using a streak camera

R. V. Krishnan, H. Saitoh, H. Terada, V. E. Centonze, B. Herman

Biomedical Engineering

Research output: Contribution to journal › Conference article › peer-review

1 Scopus citations

Abstract

We report the development, calibration and biomedical application of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (∼0.2 μm) and temporal (∼50 psec) resolution and allows rapid data acquisition and reliable and reproducible lifetime determinations. The system was calibrated with standard fluorescent dyes and the lifetime values obtained were in very good agreement with values reported in literature for these dyes. We also demonstrate the applicability of the system to FLIM studies in cellular specimens in the context of quantitative measurement of fluorescence resonance energy transfer (FRET).

Original language	English (US)
Pages (from-to)	165-174
Number of pages	10
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4963
DOIs	https://doi.org/10.1117/12.478001
State	Published - 2003
Event	Multiphoton Microscopy in the Biomedical Sciences III - San Jose,CA, United States Duration: Jan 26 2003 → Jan 28 2003

Keywords

FRET
Multiphoton FLIM
Streak camera

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10.1117/12.478001

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title = "Multiphoton fluorescence lifetime imaging microscopy (FLIM) using a streak camera",

abstract = "We report the development, calibration and biomedical application of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (∼0.2 μm) and temporal (∼50 psec) resolution and allows rapid data acquisition and reliable and reproducible lifetime determinations. The system was calibrated with standard fluorescent dyes and the lifetime values obtained were in very good agreement with values reported in literature for these dyes. We also demonstrate the applicability of the system to FLIM studies in cellular specimens in the context of quantitative measurement of fluorescence resonance energy transfer (FRET).",

keywords = "FRET, Multiphoton FLIM, Streak camera",

author = "Krishnan, {R. V.} and H. Saitoh and H. Terada and Centonze, {V. E.} and B. Herman",

year = "2003",

doi = "10.1117/12.478001",

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T1 - Multiphoton fluorescence lifetime imaging microscopy (FLIM) using a streak camera

AU - Krishnan, R. V.

AU - Saitoh, H.

AU - Terada, H.

AU - Centonze, V. E.

AU - Herman, B.

PY - 2003

Y1 - 2003

N2 - We report the development, calibration and biomedical application of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (∼0.2 μm) and temporal (∼50 psec) resolution and allows rapid data acquisition and reliable and reproducible lifetime determinations. The system was calibrated with standard fluorescent dyes and the lifetime values obtained were in very good agreement with values reported in literature for these dyes. We also demonstrate the applicability of the system to FLIM studies in cellular specimens in the context of quantitative measurement of fluorescence resonance energy transfer (FRET).

AB - We report the development, calibration and biomedical application of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (∼0.2 μm) and temporal (∼50 psec) resolution and allows rapid data acquisition and reliable and reproducible lifetime determinations. The system was calibrated with standard fluorescent dyes and the lifetime values obtained were in very good agreement with values reported in literature for these dyes. We also demonstrate the applicability of the system to FLIM studies in cellular specimens in the context of quantitative measurement of fluorescence resonance energy transfer (FRET).

KW - FRET

KW - Multiphoton FLIM

KW - Streak camera

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DO - 10.1117/12.478001

M3 - Conference article

AN - SCOPUS:0141605393

SN - 0277-786X

VL - 4963

SP - 165

EP - 174

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Multiphoton Microscopy in the Biomedical Sciences III

Y2 - 26 January 2003 through 28 January 2003

ER -

Multiphoton fluorescence lifetime imaging microscopy (FLIM) using a streak camera

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