Multiphoton fluorescence lifetime imaging microscopy (FLIM) using a streak camera

R. V. Krishnan, H. Saitoh, H. Terada, V. E. Centonze, B. Herman

Research output: Contribution to journalConference articlepeer-review


We report the development, calibration and biomedical application of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (∼0.2 μm) and temporal (∼50 psec) resolution and allows rapid data acquisition and reliable and reproducible lifetime determinations. The system was calibrated with standard fluorescent dyes and the lifetime values obtained were in very good agreement with values reported in literature for these dyes. We also demonstrate the applicability of the system to FLIM studies in cellular specimens in the context of quantitative measurement of fluorescence resonance energy transfer (FRET).

Original languageEnglish (US)
Pages (from-to)165-174
Number of pages10
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - Sep 29 2003
EventMultiphoton Microscopy in the Biomedical Sciences III - San Jose,CA, United States
Duration: Jan 26 2003Jan 28 2003


  • FRET
  • Multiphoton FLIM
  • Streak camera


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