Multilocus sequence typing of staphylococcus aureus

Ying Liu, Yinduo Ji

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Multilocus sequence typing (MLST) has been successfully used to differentiate and trace the bacterial species and pathogens that cause outbreaks or epidemics of infectious diseases. MLST provides a powerful solution for molecular epidemiological characterization of bacterial strains, including Staphylococcus aureus, by using the sequences of the internal region of seven housekeeping genes. In previous studies, we utilized MLST to analyze the genotypes of S. aureus isolates from pediatric patients with cystic fibrosis and revealed three prevalent ST types, including ST5, ST30, and ST8 in these isolates. In this chapter, we describe a detailed procedure of MLST for genotyping S. aureus.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages95-102
Number of pages8
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2069
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Multilocus Sequence Typing
Staphylococcus aureus
Essential Genes
Cystic Fibrosis
Disease Outbreaks
Communicable Diseases
Genotype
Pediatrics

Keywords

  • Genotyping
  • MLST
  • MRSA
  • Staphylococcus aureus

Cite this

Liu, Y., & Ji, Y. (2020). Multilocus sequence typing of staphylococcus aureus. In Methods in Molecular Biology (pp. 95-102). (Methods in Molecular Biology; Vol. 2069). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9849-4_7

Multilocus sequence typing of staphylococcus aureus. / Liu, Ying; Ji, Yinduo.

Methods in Molecular Biology. Humana Press Inc., 2020. p. 95-102 (Methods in Molecular Biology; Vol. 2069).

Research output: Chapter in Book/Report/Conference proceedingChapter

Liu, Y & Ji, Y 2020, Multilocus sequence typing of staphylococcus aureus. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2069, Humana Press Inc., pp. 95-102. https://doi.org/10.1007/978-1-4939-9849-4_7
Liu Y, Ji Y. Multilocus sequence typing of staphylococcus aureus. In Methods in Molecular Biology. Humana Press Inc. 2020. p. 95-102. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9849-4_7
Liu, Ying ; Ji, Yinduo. / Multilocus sequence typing of staphylococcus aureus. Methods in Molecular Biology. Humana Press Inc., 2020. pp. 95-102 (Methods in Molecular Biology).
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