Multilocus Sequence Typing of Staphylococcus aureus

Ying Liu, Yinduo Ji

Research output: Contribution to journalArticle

Abstract

Multilocus sequence typing (MLST) has been successfully used to differentiate and trace the bacterial species and pathogens that cause outbreaks or epidemics of infectious diseases. MLST provides a powerful solution for molecular epidemiological characterization of bacterial strains, including Staphylococcus aureus, by using the sequences of the internal region of seven housekeeping genes. In previous studies, we utilized MLST to analyze the genotypes of S. aureus isolates from pediatric patients with cystic fibrosis and revealed three prevalent ST types, including ST5, ST30, and ST8 in these isolates. In this chapter, we describe a detailed procedure of MLST for genotyping S. aureus.

Original languageEnglish (US)
Pages (from-to)95-102
Number of pages8
JournalMethods in molecular biology (Clifton, N.J.)
Volume2069
DOIs
StatePublished - Jan 1 2020

Fingerprint

Multilocus Sequence Typing
Staphylococcus aureus
Essential Genes
Cystic Fibrosis
Disease Outbreaks
Communicable Diseases
Genotype
Pediatrics

Keywords

  • Genotyping
  • MLST
  • MRSA
  • Staphylococcus aureus

Cite this

Multilocus Sequence Typing of Staphylococcus aureus. / Liu, Ying; Ji, Yinduo.

In: Methods in molecular biology (Clifton, N.J.), Vol. 2069, 01.01.2020, p. 95-102.

Research output: Contribution to journalArticle

@article{56ad96eb36da44c5996c762e00853b7b,
title = "Multilocus Sequence Typing of Staphylococcus aureus",
abstract = "Multilocus sequence typing (MLST) has been successfully used to differentiate and trace the bacterial species and pathogens that cause outbreaks or epidemics of infectious diseases. MLST provides a powerful solution for molecular epidemiological characterization of bacterial strains, including Staphylococcus aureus, by using the sequences of the internal region of seven housekeeping genes. In previous studies, we utilized MLST to analyze the genotypes of S. aureus isolates from pediatric patients with cystic fibrosis and revealed three prevalent ST types, including ST5, ST30, and ST8 in these isolates. In this chapter, we describe a detailed procedure of MLST for genotyping S. aureus.",
keywords = "Genotyping, MLST, MRSA, Staphylococcus aureus",
author = "Ying Liu and Yinduo Ji",
year = "2020",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-9849-4_7",
language = "English (US)",
volume = "2069",
pages = "95--102",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Multilocus Sequence Typing of Staphylococcus aureus

AU - Liu, Ying

AU - Ji, Yinduo

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Multilocus sequence typing (MLST) has been successfully used to differentiate and trace the bacterial species and pathogens that cause outbreaks or epidemics of infectious diseases. MLST provides a powerful solution for molecular epidemiological characterization of bacterial strains, including Staphylococcus aureus, by using the sequences of the internal region of seven housekeeping genes. In previous studies, we utilized MLST to analyze the genotypes of S. aureus isolates from pediatric patients with cystic fibrosis and revealed three prevalent ST types, including ST5, ST30, and ST8 in these isolates. In this chapter, we describe a detailed procedure of MLST for genotyping S. aureus.

AB - Multilocus sequence typing (MLST) has been successfully used to differentiate and trace the bacterial species and pathogens that cause outbreaks or epidemics of infectious diseases. MLST provides a powerful solution for molecular epidemiological characterization of bacterial strains, including Staphylococcus aureus, by using the sequences of the internal region of seven housekeeping genes. In previous studies, we utilized MLST to analyze the genotypes of S. aureus isolates from pediatric patients with cystic fibrosis and revealed three prevalent ST types, including ST5, ST30, and ST8 in these isolates. In this chapter, we describe a detailed procedure of MLST for genotyping S. aureus.

KW - Genotyping

KW - MLST

KW - MRSA

KW - Staphylococcus aureus

UR - http://www.scopus.com/inward/record.url?scp=85072217453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85072217453&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9849-4_7

DO - 10.1007/978-1-4939-9849-4_7

M3 - Article

VL - 2069

SP - 95

EP - 102

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -