TY - JOUR
T1 - Multiliter production and immunochemical cross‐reactivity of plant tissue culture antigens
AU - Wu, W. L.
AU - Staba, E. J.
AU - Blumenthal, Malcolm N
PY - 1976/1
Y1 - 1976/1
N2 - Cells of short ragweed suspension culture were successfully propagated in a multiliter fermentor. Proteins were isolated from tissue cultures (short ragweed, cantaloupe, marigold, and Ammi) and plants (short ragweed pollen, cantaloupe fruit, and marigold flowers) by ammonium sulfate precipitation, cross‐linked dextran gel filtration, and anion‐exchange chromatography. Some fractions elicited allergenic responses in ragweed‐sensitive patients but not in ragweed‐nonsensitive control patients. Double‐immuno‐diffusion analysis of these fractions showed their nonidentity with short ragweed antigen E.
AB - Cells of short ragweed suspension culture were successfully propagated in a multiliter fermentor. Proteins were isolated from tissue cultures (short ragweed, cantaloupe, marigold, and Ammi) and plants (short ragweed pollen, cantaloupe fruit, and marigold flowers) by ammonium sulfate precipitation, cross‐linked dextran gel filtration, and anion‐exchange chromatography. Some fractions elicited allergenic responses in ragweed‐sensitive patients but not in ragweed‐nonsensitive control patients. Double‐immuno‐diffusion analysis of these fractions showed their nonidentity with short ragweed antigen E.
KW - Antigens—proteins isolated from plant tissue cultures, allergenic activity screened
KW - Plant antigens—isolated from tissue cultures, allergenic activity screened
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U2 - 10.1002/jps.2600650122
DO - 10.1002/jps.2600650122
M3 - Article
C2 - 815539
AN - SCOPUS:0017283553
SN - 0022-3549
VL - 65
SP - 102
EP - 105
JO - Journal of Pharmaceutical Sciences
JF - Journal of Pharmaceutical Sciences
IS - 1
ER -