Cells of short ragweed suspension culture were successfully propagated in a multiliter fermentor. Proteins were isolated from tissue cultures (short ragweed, cantaloupe, marigold, and Ammi) and plants (short ragweed pollen, cantaloupe fruit, and marigold flowers) by ammonium sulfate precipitation, cross‐linked dextran gel filtration, and anion‐exchange chromatography. Some fractions elicited allergenic responses in ragweed‐sensitive patients but not in ragweed‐nonsensitive control patients. Double‐immuno‐diffusion analysis of these fractions showed their nonidentity with short ragweed antigen E.
- Antigens—proteins isolated from plant tissue cultures, allergenic activity screened
- Plant antigens—isolated from tissue cultures, allergenic activity screened