Multiliter production and immunochemical cross‐reactivity of plant tissue culture antigens

W. L. Wu, E. J. Staba, Malcolm N Blumenthal

Research output: Contribution to journalArticlepeer-review

Abstract

Cells of short ragweed suspension culture were successfully propagated in a multiliter fermentor. Proteins were isolated from tissue cultures (short ragweed, cantaloupe, marigold, and Ammi) and plants (short ragweed pollen, cantaloupe fruit, and marigold flowers) by ammonium sulfate precipitation, cross‐linked dextran gel filtration, and anion‐exchange chromatography. Some fractions elicited allergenic responses in ragweed‐sensitive patients but not in ragweed‐nonsensitive control patients. Double‐immuno‐diffusion analysis of these fractions showed their nonidentity with short ragweed antigen E.

Original languageEnglish (US)
Pages (from-to)102-105
Number of pages4
JournalJournal of Pharmaceutical Sciences
Volume65
Issue number1
DOIs
StatePublished - Jan 1976

Keywords

  • Antigens—proteins isolated from plant tissue cultures, allergenic activity screened
  • Plant antigens—isolated from tissue cultures, allergenic activity screened

Fingerprint Dive into the research topics of 'Multiliter production and immunochemical cross‐reactivity of plant tissue culture antigens'. Together they form a unique fingerprint.

Cite this