Multifactorial Sex Determination in Chameleons

Cissy J. Ballen, Richard Shine, Robin M. Andrews, Mats Olsson

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Classifying a species as having either genotypic or environmental sex determination may oversimplify the processes that influence offspring sex. Using two independently gathered data sets on veiled chameleons (Chamaeleo calyptratus), we show that a hatchling's sex is affected by the interaction between egg mass and incubation temperature. In both studies, larger eggs produced daughters at lower temperatures and sons at higher temperatures; however, the relationships between incubation temperature, egg mass, and sex diverged markedly between the two studies. The shift in egg-size effects was seen between 25°C and 28°C in one study and between 28°C and 30°C in the other. The links between offspring size, sex, and incubation temperature are not an artifact of differential mortality; in one of the studies, egg survival rates were uniformly high. Other data on scincid and agamid lizards also do not support a simple dichotomy in sex-determining systems. Our results challenge simplistic classificatory schemes and call for a reanalysis of existing data sets to look for multifactorial as well as unifactorial effects on offspring sex.

Original languageEnglish (US)
Pages (from-to)548-551
Number of pages4
JournalJournal of Herpetology
Issue number4
StatePublished - Dec 1 2016

Bibliographical note

Funding Information:
We thank the Australian Research Council and the University of Sydney for funding and M. Elphick for her help and support. All work was carried out under the approval of the University of Sydney Animal Ethics Committee (permit L04/4-2012/3/5698).

Publisher Copyright:
© Copyright 2016 Society for the Study of Amphibians and Reptiles.


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