Multicolor Fluorescence Microscopy Using the Laser-Scanning Confocal Microscope

Martin W. Wessendorf, T. Clark Brelje

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Neurons and glia can be labeled using fluorescence immunocytochemistry, fluorescent tract-tracers, and a variety of other techniques that use fluorescence. Combining these methods to label tissue with two or more fluorescent probes can increase the power of an experimental design. The present paper examines the issues related to the use of laser-scanning confocal microscopy to image combinations of fluorescent labels and recommends specific combinations of lasers, filters, and fluorophores.

Original languageEnglish (US)
Pages (from-to)121-140
Number of pages20
JournalNeuroprotocols
Volume2
Issue number2
DOIs
StatePublished - Apr 1 1993

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