Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning

T. Gopinath; Daniel K. Weber; Gianluigi Veglia

doi:10.1007/s10858-020-00316-y

Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning

T. Gopinath, Daniel K. Weber, Gianluigi Veglia

Chemical and Structural Biology (TMED)

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Ultrafast magic angle spinning (MAS) technology and ¹H detection have dramatically enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We previously showed that, when combined with polarization optimized experiments (POE), these advancements enable the simultaneous acquisition of multi-dimensional ¹H- or ¹³C-detected experiments using a single receiver. Here, we propose a new sub-class within the POE family, namely HC-DUMAS, HC-MEIOSIS, and HC-MAeSTOSO, that utilize dual receiver technology for the simultaneous detection of ¹H and ¹³C nuclei. We also expand this approach to record ¹H-, ¹³C-, and ¹⁵N-detected homonuclear 2D spectra simultaneously using three independent receivers. The combination of POE and multi-receiver technology will further shorten the total experimental time of ssNMR experiments for biological solids.

Original language	English (US)
Pages (from-to)	267-285
Number of pages	19
Journal	Journal of biomolecular NMR
Volume	74
Issue number	4-5
DOIs	https://doi.org/10.1007/s10858-020-00316-y
State	Published - May 1 2020

Bibliographical note

Funding Information:
This work was supported by the National Institute of Health (GM 64742, HL 144130, 1S10OD021536 to G.V.), the American Heart Association (19POST34420009 to D.W.), and the Minnesota NMR Center. We also thank Dr. J. Struppe and Dr. S. Pawsey from Bruker R&D for helpful discussions.

Publisher Copyright:
© 2020, Springer Nature B.V.

Keywords

HC-DUMAS
HC-MAeSTOSO
HC-MEIOSIS
Multi-acquisition
Multi-receiver
Polarization optimized experiments (POE)
SIM-CP
Solid-state NMR
Ultra-fast magic angle spinning

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abstract = "Ultrafast magic angle spinning (MAS) technology and 1H detection have dramatically enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We previously showed that, when combined with polarization optimized experiments (POE), these advancements enable the simultaneous acquisition of multi-dimensional 1H- or 13C-detected experiments using a single receiver. Here, we propose a new sub-class within the POE family, namely HC-DUMAS, HC-MEIOSIS, and HC-MAeSTOSO, that utilize dual receiver technology for the simultaneous detection of 1H and 13C nuclei. We also expand this approach to record 1H-, 13C-, and 15N-detected homonuclear 2D spectra simultaneously using three independent receivers. The combination of POE and multi-receiver technology will further shorten the total experimental time of ssNMR experiments for biological solids.",

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author = "T. Gopinath and Weber, {Daniel K.} and Gianluigi Veglia",

note = "Funding Information: This work was supported by the National Institute of Health (GM 64742, HL 144130, 1S10OD021536 to G.V.), the American Heart Association (19POST34420009 to D.W.), and the Minnesota NMR Center. We also thank Dr. J. Struppe and Dr. S. Pawsey from Bruker R&D for helpful discussions. Publisher Copyright: {\textcopyright} 2020, Springer Nature B.V.",

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N2 - Ultrafast magic angle spinning (MAS) technology and 1H detection have dramatically enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We previously showed that, when combined with polarization optimized experiments (POE), these advancements enable the simultaneous acquisition of multi-dimensional 1H- or 13C-detected experiments using a single receiver. Here, we propose a new sub-class within the POE family, namely HC-DUMAS, HC-MEIOSIS, and HC-MAeSTOSO, that utilize dual receiver technology for the simultaneous detection of 1H and 13C nuclei. We also expand this approach to record 1H-, 13C-, and 15N-detected homonuclear 2D spectra simultaneously using three independent receivers. The combination of POE and multi-receiver technology will further shorten the total experimental time of ssNMR experiments for biological solids.

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ER -

Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning

Abstract

Bibliographical note

Keywords

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