Multi-probe RPA template sets to study RNA modulation and transcriptional control of BH3-only members of the Bcl-2 family

Dehua Wang, Maureen A. McDonnell, Ameeta Kelekar

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The Bcl-2 family of apoptotic regulators comprises both pro- and anti-apoptotic proteins. Pro-apoptotic BH3-only members of the Bcl-2 family are subject to stringent control in normal proliferating cells, but can be activated by a variety of mechanisms in response to diverse apoptotic signals. To facilitate the simultaneous identification of BH3-only targets that are regulated at the RNA level, we have designed and assembled multi-probe ribonuclease protection assay templates and demonstrated their ability to detect multiple BH3-only transcripts in a variety of human cell lines and primary tissues. Following this, we have determined the modulation of BH3-only transcripts to a variety of apoptotic stimuli, in selected primary and transformed cell lines. Early experiments, showing that a given cell type upregulates a unique subset of BH3-only RNAs in response to different death-inducing stimuli, indicate that the multi-probe BH3-only RPA template sets could serve as useful diagnostic and analytical tools in cancer research.

Original languageEnglish (US)
Pages (from-to)189-200
Number of pages12
JournalCancer Detection and Prevention
Volume29
Issue number2
DOIs
StatePublished - 2005

Bibliographical note

Funding Information:
This study was supported by a grant (3051-9227-01) from the Minnesota Medical Foundation.

Keywords

  • Apoptosis
  • BH3-only proteins
  • Bcl-2 family
  • RNase protection assay
  • Transcriptional control

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