Multi-chamber microfluidic platform for high-precision skin permeation testing

M. Alberti, Y. Dancik, G. Sriram, B. Wu, Y. L. Teo, Z. Feng, M. Bigliardi-Qi, R. G. Wu, Z. P. Wang, P. L. Bigliardi

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The established in vitro tool used for testing the absorption and penetration of chemicals through skin in pharmacology, toxicology and cosmetic science is the static Franz diffusion cell. While widespread, Franz cells are relatively costly, low-throughput and results may suffer from poor reproducibility. Microfluidics has the potential to overcome these drawbacks. In this paper, we present a novel microfluidic skin permeation platform and validate it rigorously against the Franz cell by comparing the transport of 3 model chemicals of varying lipophilicity: caffeine, salicylic acid and testosterone. Permeation experiments through silicone membranes show that the chip yields higher sensitivity in permeant cumulative amounts and comparable or lower coefficients of variation. Using a skin organotypic culture, we show that the chip decreases the effect of unstirred water layers that can occur in static Franz cells. The validation reported herein sets the stage for efficient skin permeation and toxicity screening and further development of microfluidic skin-on-chip devices.

Original languageEnglish (US)
Pages (from-to)1625-1634
Number of pages10
JournalLab on a chip
Volume17
Issue number9
DOIs
StatePublished - Jan 1 2017

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Alberti, M., Dancik, Y., Sriram, G., Wu, B., Teo, Y. L., Feng, Z., Bigliardi-Qi, M., Wu, R. G., Wang, Z. P., & Bigliardi, P. L. (2017). Multi-chamber microfluidic platform for high-precision skin permeation testing. Lab on a chip, 17(9), 1625-1634. https://doi.org/10.1039/c6lc01574c