mRNA and protein levels for GABA Aα4, α5, β1 and GABABR1 receptors are altered in brains from subjects with autism

S. Hossein Fatemi, Teri J. Reutiman, Timothy D. Folsom, Robert J. Rooney, Diven H. Patel, Paul D. Thuras

Research output: Contribution to journalArticlepeer-review

125 Scopus citations

Abstract

We have shown altered expression of gammaaminobutyric acid A (GABA A) and gamma-aminobutyric acid B (GABA B) receptors in the brains of subjects with autism. In the current study, we sought to verify our western blotting data for GABBR1 via qRT-PCR and to expand our previous work to measure mRNA and protein levels of 3 GABA A subunits previously associated with autism (GABRα4; GABRα5; GABRβ1). Three GABA receptor subunitsdemonstrated mRNA and protein level concordance in superior frontal cortex (GABRα4, GABRα5, GABRβ1) and one demonstrated concordance in cerebellum (GABBR1). These results provide further evidence of impairment of GABAergic signaling in autism.

Original languageEnglish (US)
Pages (from-to)743-750
Number of pages8
JournalJournal of Autism and Developmental Disorders
Volume40
Issue number6
DOIs
StatePublished - Jun 2010

Bibliographical note

Funding Information:
Acknowledgments Human tissue was obtained from the NICHD Brain and Tissue Bank for Developmental Disorders; the Harvard Brain Tissue Resource Center, which is supported in part by PHS grant number R24 MH068855; the Brain Endowment Bank, which is funded in part by the National Parkinson Foundation, Inc., Miami, Florida; and the Autism Tissue Program and is gratefully acknowledged. Grant support by National Institute of Child Health and Human Development (#5R01HD052074-01A2) and 3R01HD052074-03S1 to SHF is gratefully acknowledged. Portions of this paper have been presented at the following meetings: Collegium Internationale Neuro-Psychopharma-cologicum, Munich, 2008; American College of Neuropsychophar-macology, Scottsdale AZ, 2008; University of Tehran, School of Medicine, 2008; Autism One Conference, Chicago, 2009.

Funding Information:
All experimental procedures were approved by the Institutional Review Board of the University of Minnesota School of Medicine. Postmortem blocks of BA40, BA9, and cerebella (lobar origin unknown) were obtained from the Autism Research Foundation and various brain banks (NICHD Brain and Tissue Bank for Developmental Disorders; TARF; the Harvard Brain Tissue Resource Center, which is supported in part by PHS grant number R24 MH068855; the Brain Endowment Bank, which is funded in part by the National Parkinson Foundation, Inc., Miami, Florida; and the Autism Tissue Program). The tissue

Keywords

  • Autism
  • Brain
  • GABBR1
  • GABRα4
  • GABRα5
  • GABRβ1

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