Mouse xenograft model for intraperitoneal administration of NK cell immunotherapy for ovarian cancer

David L. Hermanson, Laura Bendzick, Dan S. Kaufman

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Natural killer (NK) cells are an attractive cell population for immunotherapy. Adoptive transfer of NK cells has been tested in multiple clinical trials including acute myeloid leukemia (AML) and ovarian cancer, although limitations do exist especially for treatment of solid tumors. In order to overcome these limitations, mouse xenograft models are needed for evaluation of various NK cell populations, as well as routes of NK cell administration. Here, we describe the methods used for the establishment of an intraperitoneal (ip) ovarian cancer mouse xenograft model with ip delivery of NK cells. This model has been successfully employed with multiple ovarian cell lines and could be applied to other tumor models where the tumor’s primary location is in the peritoneal cavity. It is also compatible with multiple routes of NK cell administration. Bioluminescent imaging for monitoring tumor formation and response provides for easy visualization of NK cell tumor inhibition. This xenograft model is superior to other models because the tumor is implanted into the same physiological space where ovarian cancer is found, which allows for improved mimicking of actual disease.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages277-284
Number of pages8
DOIs
StatePublished - 2016

Publication series

NameMethods in Molecular Biology
Volume1441
ISSN (Print)1064-3745

Keywords

  • Bioluminescent imaging
  • Immunotherapy
  • Mouse xenograft
  • Natural killer cells
  • Ovarian cancer

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