Mouse Thymocyte Membrane Uptake of Polyadenylic-Polyuridylic Acid Complexes

Milton G. Mutchnick; Ihn H. Han; Arthur G. Johnson

doi:10.3181/00379727-154-39651

Mouse Thymocyte Membrane Uptake of Polyadenylic-Polyuridylic Acid Complexes

Milton G. Mutchnick, Ihn H. Han, Arthur G. Johnson

Biomedical Sciences

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7 Scopus citations

Abstract

Indirect immunofluorescence revealed that 80 ± 4% of isolated mouse thymocytes were found to have membrane uptake of poly (A:U). Mice previously injected with hydrocortisone demonstrated a reduction to 20-30% in numbers of fluorescent thymocytes. Preincubation of thymocytes with DEAE-dextran did not increase the percentage of fluorescent cells. These findings suggest that medullary thymocytes bind poly (A:U) to a lesser degree than cortical thymocytes. Several mechanisms of thymocyte uptake of poly (A:U) are suggested including adsorption to the membrane and/or rapid penetration through the membrane.

Original language	English (US)
Pages (from-to)	264-268
Number of pages	5
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	154
Issue number	2
DOIs	https://doi.org/10.3181/00379727-154-39651
State	Published - Feb 1977

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abstract = "Indirect immunofluorescence revealed that 80 ± 4% of isolated mouse thymocytes were found to have membrane uptake of poly (A:U). Mice previously injected with hydrocortisone demonstrated a reduction to 20-30% in numbers of fluorescent thymocytes. Preincubation of thymocytes with DEAE-dextran did not increase the percentage of fluorescent cells. These findings suggest that medullary thymocytes bind poly (A:U) to a lesser degree than cortical thymocytes. Several mechanisms of thymocyte uptake of poly (A:U) are suggested including adsorption to the membrane and/or rapid penetration through the membrane.",

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N2 - Indirect immunofluorescence revealed that 80 ± 4% of isolated mouse thymocytes were found to have membrane uptake of poly (A:U). Mice previously injected with hydrocortisone demonstrated a reduction to 20-30% in numbers of fluorescent thymocytes. Preincubation of thymocytes with DEAE-dextran did not increase the percentage of fluorescent cells. These findings suggest that medullary thymocytes bind poly (A:U) to a lesser degree than cortical thymocytes. Several mechanisms of thymocyte uptake of poly (A:U) are suggested including adsorption to the membrane and/or rapid penetration through the membrane.

AB - Indirect immunofluorescence revealed that 80 ± 4% of isolated mouse thymocytes were found to have membrane uptake of poly (A:U). Mice previously injected with hydrocortisone demonstrated a reduction to 20-30% in numbers of fluorescent thymocytes. Preincubation of thymocytes with DEAE-dextran did not increase the percentage of fluorescent cells. These findings suggest that medullary thymocytes bind poly (A:U) to a lesser degree than cortical thymocytes. Several mechanisms of thymocyte uptake of poly (A:U) are suggested including adsorption to the membrane and/or rapid penetration through the membrane.

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Mouse Thymocyte Membrane Uptake of Polyadenylic-Polyuridylic Acid Complexes

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