Mouse Models of Pain in Sickle Cell Disease

Varun Sagi, Waogwende L. Song-Naba, Barbara A. Benson, Sonal S. Joshi, Kalpna Gupta

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Sickle cell disease (SCD) is a genetic blood disorder that impacts millions of individuals worldwide. SCD is characterized by debilitating pain that can begin during infancy and may continue to increase throughout life. This pain can be both acute and chronic. A characteristic feature specific to acute pain in SCD occurs during vaso-occlusive crisis (VOC) due to the blockade of capillaries with sickle red blood cells. The acute pain of VOC is intense, unpredictable, and requires hospitalization. Chronic pain occurs in a significant population with SCD. Treatment options for sickle pain are limited and primarily involve the use of opioids. However, long-term opioid use is associated with numerous side effects. Thus, pain management in SCD remains a major challenge. Humanized transgenic mice expressing exclusively human sickle hemoglobin show features of pain and pathobiology similar to that in patients with SCD. Therefore, these mice offer the potential for investigating the mechanisms of pain in SCD and allow for development of novel targeted analgesic therapies.

Original languageEnglish (US)
Article numbere54
JournalCurrent Protocols in Neuroscience
Volume85
Issue number1
DOIs
StatePublished - Oct 2018

Bibliographical note

Funding Information:
We thank Ms. Ritu Jha and Stacy Kivens for providing input on procedural details for breeding sickle mice and Huy Tran for input on pain measures. This work is supported by NIH/NHLBI U01HL117664. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

Funding Information:
We thank Ms. Ritu Jha and Stacy Kivens for providing input on procedural details for breeding sickle mice and Huy Tran for input on pain measures. This work is supported by NIH/NHLBI U01HL117664. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. VS wrote the protocol and prepared the illustrations and manuscript for submission; BB wrote the protocol; WLS-N co-wrote the pain measures; SJ co-wrote the introduction and background; KG designed, supervised, and edited the protocol.

Publisher Copyright:
© 2018 John Wiley & Sons, Inc.

Keywords

  • cellulose acetate electrophoresis
  • hyperalgesia
  • hypoxia/reoxygenation
  • isoelectric focusing
  • pain
  • sickle cell disease
  • vaso-occlusive crises

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