TY - JOUR
T1 - Mouse Ly49G2+ NK cells dominate early responses during both immune reconstitution and activation independently of MHC
AU - Barao, Isabel
AU - Alvarez, Maite
AU - Ames, Erik
AU - Orr, Mark T.
AU - Stefanski, Heather E.
AU - Blazar, Bruce R.
AU - Lanier, Lewis L.
AU - Anderson, Stephen K.
AU - Redelman, Doug
AU - Murphy, William J.
PY - 2011/6/30
Y1 - 2011/6/30
N2 - Natural killer (NK) cell subsets can be defined by the differential expression of inhibitory receptors for MHC class I molecules. Early after congenic HSCT, we found that Ly49G2high single-positive NK cells repopulated, displayed an activated phenotype, and were highly cytolytic. Over time, this subset was replaced with NK cells with a normal pattern of Ly49 expression. Treatment of mice with IL-2 also resulted in the rapid expansion of these Ly49G2high single-positive NK cells. Only the Ly49g (Klra7) Pro1 transcript was highly induced in both HSCT- and IL-2-treated recipients. MHC-independent expansion of the Ly49G2+ subset was also observed after Listeria monocytogenes or mouse cytomegalovirus infection. Our data indicate that during reconstitution after HSCT and various activation stimuli, Ly49G2+ NK cells represent the "first-responder" NK cells, which occur independently of NK-cell licensing via Ly49-MHC interactions. These data suggest that the inhibitory Ly49G2 receptor represents an activation marker on mouse NK cells under various conditions.
AB - Natural killer (NK) cell subsets can be defined by the differential expression of inhibitory receptors for MHC class I molecules. Early after congenic HSCT, we found that Ly49G2high single-positive NK cells repopulated, displayed an activated phenotype, and were highly cytolytic. Over time, this subset was replaced with NK cells with a normal pattern of Ly49 expression. Treatment of mice with IL-2 also resulted in the rapid expansion of these Ly49G2high single-positive NK cells. Only the Ly49g (Klra7) Pro1 transcript was highly induced in both HSCT- and IL-2-treated recipients. MHC-independent expansion of the Ly49G2+ subset was also observed after Listeria monocytogenes or mouse cytomegalovirus infection. Our data indicate that during reconstitution after HSCT and various activation stimuli, Ly49G2+ NK cells represent the "first-responder" NK cells, which occur independently of NK-cell licensing via Ly49-MHC interactions. These data suggest that the inhibitory Ly49G2 receptor represents an activation marker on mouse NK cells under various conditions.
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U2 - 10.1182/blood-2010-11-316653
DO - 10.1182/blood-2010-11-316653
M3 - Article
C2 - 21498673
AN - SCOPUS:79959832208
SN - 0006-4971
VL - 117
SP - 7032
EP - 7041
JO - Blood
JF - Blood
IS - 26
ER -