We report the sequence and propose a secondary structure for the cytoplasmic large subunit (5.8S and 28S) ribosomal RNA of the mosquito, Aedes albopictus, in an aligned format that incorporates secondary structure comparisons with Homo sapiens, Drosophila melanogaster, and Escherichia coli ribosomal RNAs. This format facilitates comparison of subtle differences between models, allowing nucleotide by nucleotide analysis at each position of discrepancy. Comparison of the A. albopictus large subunit ribosomal RNA gene with those from other species revealed new compensatory base changes. The aligned format focuses attention to the specific contribution of the A. albopictus sequence by facilitating comparison with the sequence of another dipteran, D. melanogaster. This is the second report of a complete large subunit rRNA sequence from an arthropod, and the first 28S rRNA sequence for a member of the lower Diptera (Nematocera).
Bibliographical noteFunding Information:
This work was supported by the National Institutes of Health grant AI 20385, the Alexander P. and Lydia Anderson Fellowship (K.M.K.) and by the University of Minnesota Agricultural Experiment Station. This is publication 20,077 from the University of Minnesota experiment station, St. Paul, MN.
- RNA structure
- Ribosomal RNA
- Secondary structure
- rRNA sequence