Morphology and growth potential of stromal cell cultures derived from human endometrium

B. Hugh Dorman, V. A. Varma, Jill M. Siegfried, Susan A. Melin, Thomas A. Admaec, Carol R. Norton, David G. Kaufman

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Propagable cell cultures derived from human endometrial tissue were determined to contain cells predominantly of stromal cell origin based on their morphologic resemblance to endometrial stromal cells. These features included nexi, solitary cilia, and predecidual cytology. In addition to morphology the cell cultures retained a normal karyotype and responded to steroid hormones as evidenced by cellular aggregation. The stromal cells were evaluated for a variety of characteristics associated with transformed cells and seemed to be biologically normal without neoplastic phenotypes. Growth potential of the stromal cell cultures was also characterized in normal maintenance medium, in nutritionally depleted medium with reduced levels of calcium or serum, and in medium with increased levels of serum. The prolonged survival of the stromal cells in vitro coupled with the retention of in vivo characteristics and an absence of neoplastic phenotype provides a human cell system that is amenable to a variety of long-term experimental analyses.

Original languageEnglish (US)
Pages (from-to)919-928
Number of pages10
JournalIn Vitro
Volume18
Issue number11
DOIs
StatePublished - Nov 1982

Keywords

  • human endometrium
  • stromal cell

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