Morphologic evaluation and integrin expression profile of renal tubular cells cultured from percutaneous renal biopsy specimen

Jung Sim Kim, Hye Zin Hwang, Sung Hee Yeo, Shi Whan Ko, Seng Mi Song, Yoon Ku Kim, Dae Jung Kim, Ha Young Oh, Han Yong Choi, Mi Kyung Kim, Shibata Toru, Takao Kohsaka, Youngki Kim, Dong Kyu Jin

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Kidney biopsy is an indispensible procedure for making a pathologic diagnosis of renal diseases by fixing and staining the biopsy specimen. However, it is not a routine procedure to culture the cells from a renal biopsy specimen directly, or to utilize the cultured cells for any kind of diagnostic or functional evaluation. In this study, primary culture of the renal tubular epithelial cells was tried from a piece of percutaneous kidney biopsy specimen. Successive passages of the cells were possible until fourth passage. With these cells, morphologic characteristics of the cultured cells and integrin expression profiles were investigated. On light and electron microscopy, these cells were characterized by the cobblestone-like growth, presence of microvilli and tight junction, and the preservation of polarity. Immunohistochemical studies demonstrated the epithelial nature of these cells and particularly their differentiation from renal tubular epithelial cells, of either proximal or distal nephronic segment. The integrin profile confirms the epithelial nature of the cell. We hope that our results facilitate the understanding of pathophysiology of renal tubular cells from the patient directly.

Original languageEnglish (US)
Pages (from-to)21-29
Number of pages9
JournalRenal Failure
Volume23
Issue number1
DOIs
StatePublished - 2001

Bibliographical note

Funding Information:
This work was supported by grants (#HMP-97-B-3-0030) from the 97 Good Health R&D project, Ministry of Health & Welfare, R.O.K., and Samsung grant #SBRI C-96-018.

Keywords

  • Cell culture
  • Integrin
  • Kidney biopsy
  • Proximal tubular epithelial cell

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