Morphine inhibits the release of tumor necrosis factor in human peripheral blood mononuclear cell cultures

Chun C. Chao, Thomas W Molitor, Karen Close, Shuxian Hu, Phillip K. Peterson

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Opiates modulate a variety of immune responses of peripheral blood mononuclear cells (PBMC). When PBMC were treated with morphine for 24 h, cells released less (P<0.05) bioactive TNF, a cytokine important in host defense, in the following 24-h incubation period when stimulated with lipopolysaccharide and phytohemagglutinin. Morphine alone did not significantly alter the release of TNF from PBMC cultures. Pretreatment of PBMC cultures for 1 h with naloxone blocked (P<0.05) the inhibitory effect of morphine on the release of TNF upon stimulation with phytohemagglutinin, but not with lipopolysaccharide, suggesting the involvement of an opioid receptor. The mechanism of morphine-induced suppresion of TNF release appears to be counteracted by the effect of this opiate on the release of transforming growth factor (TGF)-β, since antibodies to this immunoregulatory cytokine further enhanced morphine-related inhibition of TNF release. Taken together, these findings indicate that morphine suppresses the release of bioactive TNF from PBMC and that TGF-β plays a modulatory role in this inhibitory process.

Original languageEnglish (US)
Pages (from-to)447-453
Number of pages7
JournalInternational Journal of Immunopharmacology
Issue number3
StatePublished - Apr 1993

Bibliographical note

Funding Information:
Acknowledgements -- The authors thank Monica Tsang for providing antibodies. This work was supported in part by U.S. Public Health Service Grant DA-04381.

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