TY - JOUR
T1 - Morphine inhibits the release of tumor necrosis factor in human peripheral blood mononuclear cell cultures
AU - Chao, Chun C.
AU - Molitor, Thomas W
AU - Close, Karen
AU - Hu, Shuxian
AU - Peterson, Phillip K.
N1 - Funding Information:
Acknowledgements -- The authors thank Monica Tsang for providing antibodies. This work was supported in part by U.S. Public Health Service Grant DA-04381.
PY - 1993/4
Y1 - 1993/4
N2 - Opiates modulate a variety of immune responses of peripheral blood mononuclear cells (PBMC). When PBMC were treated with morphine for 24 h, cells released less (P<0.05) bioactive TNF, a cytokine important in host defense, in the following 24-h incubation period when stimulated with lipopolysaccharide and phytohemagglutinin. Morphine alone did not significantly alter the release of TNF from PBMC cultures. Pretreatment of PBMC cultures for 1 h with naloxone blocked (P<0.05) the inhibitory effect of morphine on the release of TNF upon stimulation with phytohemagglutinin, but not with lipopolysaccharide, suggesting the involvement of an opioid receptor. The mechanism of morphine-induced suppresion of TNF release appears to be counteracted by the effect of this opiate on the release of transforming growth factor (TGF)-β, since antibodies to this immunoregulatory cytokine further enhanced morphine-related inhibition of TNF release. Taken together, these findings indicate that morphine suppresses the release of bioactive TNF from PBMC and that TGF-β plays a modulatory role in this inhibitory process.
AB - Opiates modulate a variety of immune responses of peripheral blood mononuclear cells (PBMC). When PBMC were treated with morphine for 24 h, cells released less (P<0.05) bioactive TNF, a cytokine important in host defense, in the following 24-h incubation period when stimulated with lipopolysaccharide and phytohemagglutinin. Morphine alone did not significantly alter the release of TNF from PBMC cultures. Pretreatment of PBMC cultures for 1 h with naloxone blocked (P<0.05) the inhibitory effect of morphine on the release of TNF upon stimulation with phytohemagglutinin, but not with lipopolysaccharide, suggesting the involvement of an opioid receptor. The mechanism of morphine-induced suppresion of TNF release appears to be counteracted by the effect of this opiate on the release of transforming growth factor (TGF)-β, since antibodies to this immunoregulatory cytokine further enhanced morphine-related inhibition of TNF release. Taken together, these findings indicate that morphine suppresses the release of bioactive TNF from PBMC and that TGF-β plays a modulatory role in this inhibitory process.
UR - http://www.scopus.com/inward/record.url?scp=0027229191&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027229191&partnerID=8YFLogxK
U2 - 10.1016/0192-0561(93)90057-6
DO - 10.1016/0192-0561(93)90057-6
M3 - Article
C2 - 8389331
AN - SCOPUS:0027229191
VL - 15
SP - 447
EP - 453
JO - International Immunopharmacology
JF - International Immunopharmacology
SN - 1567-5769
IS - 3
ER -