Morphine amplifies HIV-1 expression in chronically infected promonocytes cocultured with human brain cells

Phillip K. Peterson; Genya Gekker; Shuxian Hu; W. Robert Anderson; Frederick Kravitz; Philip S. Portoghese; Henry H. Balfour; Chun C. Chao

doi:10.1016/0165-5728(94)90043-4

Morphine amplifies HIV-1 expression in chronically infected promonocytes cocultured with human brain cells

Phillip K. Peterson
, Genya Gekker
, Shuxian Hu
, W. Robert Anderson
, Frederick Kravitz
, Philip S. Portoghese
, Henry H. Balfour
, Chun C. Chao

Research output: Contribution to journal › Article › peer-review

111 Scopus citations

Abstract

Previous studies have shown that morphine promotes the replication of human immunodeficiency virus (HIV)-1 in peripheral blood mononuclear cell cocultures. In the present study, we tested the hypothesis that morphine would amplify HIV-1 expression in the chronically infected promonocytic clone U1 when cocultured with lipopolysaccharide-stimulated human fetal brain cells. Marked upregulation of HIV-1 expression was observed in these cocultures (quantified by measurement of HIV-1 p24 antigen levels in supernatants), and treatment of brain cells with morphine resulted in a bell-shaped dose-dependent enhancement of viral expression. The mechanism of morphine's amplifying effect appears to be opioid receptor-mediated and to involve enhanced production of tumor necrosis factor-α by microglial cells.

Original language	English (US)
Pages (from-to)	167-175
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	50
Issue number	2
DOIs	https://doi.org/10.1016/0165-5728(94)90043-4
State	Published - Mar 1994

Bibliographical note

Funding Information:
We thank Dr. StephenC ruikshankfo r his advice, Dr. Monica Tsang for providinga ntibodiest o cytokines,a nd JacquelineO stroumf or help in preparation of the manuscriptT.h is work was supportedin part by United StatesP ublic Health ServicesG rants DA-04381,T 32-DA-07239a, nd AI-27661 and a grant from HennepinF acultyA ssociates.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Human brain cell culture
Human immunodeficiency virus-1
Microglia
Morphine
Tumor necrosis- factor-α

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10.1016/0165-5728(94)90043-4

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title = "Morphine amplifies HIV-1 expression in chronically infected promonocytes cocultured with human brain cells",

abstract = "Previous studies have shown that morphine promotes the replication of human immunodeficiency virus (HIV)-1 in peripheral blood mononuclear cell cocultures. In the present study, we tested the hypothesis that morphine would amplify HIV-1 expression in the chronically infected promonocytic clone U1 when cocultured with lipopolysaccharide-stimulated human fetal brain cells. Marked upregulation of HIV-1 expression was observed in these cocultures (quantified by measurement of HIV-1 p24 antigen levels in supernatants), and treatment of brain cells with morphine resulted in a bell-shaped dose-dependent enhancement of viral expression. The mechanism of morphine's amplifying effect appears to be opioid receptor-mediated and to involve enhanced production of tumor necrosis factor-α by microglial cells.",

keywords = "Human brain cell culture, Human immunodeficiency virus-1, Microglia, Morphine, Tumor necrosis- factor-α",

author = "Peterson, \{Phillip K.\} and Genya Gekker and Shuxian Hu and Anderson, \{W. Robert\} and Frederick Kravitz and Portoghese, \{Philip S.\} and Balfour, \{Henry H.\} and Chao, \{Chun C.\}",

note = "Funding Information: We thank Dr. StephenC ruikshankfo r his advice, Dr. Monica Tsang for providinga ntibodiest o cytokines,a nd JacquelineO stroumf or help in preparation of the manuscriptT.h is work was supportedin part by United StatesP ublic Health ServicesG rants DA-04381,T 32-DA-07239a, nd AI-27661 and a grant from HennepinF acultyA ssociates.",

year = "1994",

month = mar,

doi = "10.1016/0165-5728(94)90043-4",

language = "English (US)",

volume = "50",

pages = "167--175",

journal = "Journal of Neuroimmunology",

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publisher = "Elsevier BV",

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T1 - Morphine amplifies HIV-1 expression in chronically infected promonocytes cocultured with human brain cells

AU - Peterson, Phillip K.

AU - Gekker, Genya

AU - Hu, Shuxian

AU - Anderson, W. Robert

AU - Kravitz, Frederick

AU - Portoghese, Philip S.

AU - Balfour, Henry H.

AU - Chao, Chun C.

N1 - Funding Information: We thank Dr. StephenC ruikshankfo r his advice, Dr. Monica Tsang for providinga ntibodiest o cytokines,a nd JacquelineO stroumf or help in preparation of the manuscriptT.h is work was supportedin part by United StatesP ublic Health ServicesG rants DA-04381,T 32-DA-07239a, nd AI-27661 and a grant from HennepinF acultyA ssociates.

PY - 1994/3

Y1 - 1994/3

N2 - Previous studies have shown that morphine promotes the replication of human immunodeficiency virus (HIV)-1 in peripheral blood mononuclear cell cocultures. In the present study, we tested the hypothesis that morphine would amplify HIV-1 expression in the chronically infected promonocytic clone U1 when cocultured with lipopolysaccharide-stimulated human fetal brain cells. Marked upregulation of HIV-1 expression was observed in these cocultures (quantified by measurement of HIV-1 p24 antigen levels in supernatants), and treatment of brain cells with morphine resulted in a bell-shaped dose-dependent enhancement of viral expression. The mechanism of morphine's amplifying effect appears to be opioid receptor-mediated and to involve enhanced production of tumor necrosis factor-α by microglial cells.

AB - Previous studies have shown that morphine promotes the replication of human immunodeficiency virus (HIV)-1 in peripheral blood mononuclear cell cocultures. In the present study, we tested the hypothesis that morphine would amplify HIV-1 expression in the chronically infected promonocytic clone U1 when cocultured with lipopolysaccharide-stimulated human fetal brain cells. Marked upregulation of HIV-1 expression was observed in these cocultures (quantified by measurement of HIV-1 p24 antigen levels in supernatants), and treatment of brain cells with morphine resulted in a bell-shaped dose-dependent enhancement of viral expression. The mechanism of morphine's amplifying effect appears to be opioid receptor-mediated and to involve enhanced production of tumor necrosis factor-α by microglial cells.

KW - Human brain cell culture

KW - Human immunodeficiency virus-1

KW - Microglia

KW - Morphine

KW - Tumor necrosis- factor-α

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SN - 0165-5728

VL - 50

SP - 167

EP - 175

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

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Morphine amplifies HIV-1 expression in chronically infected promonocytes cocultured with human brain cells

Abstract

Bibliographical note

UN SDGs

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