More than a monolayer: Relating lung surfactant structure and mechanics to composition

Coralie Alonso, Tim Alig, Joonsung Yoon, Frank Bringezu, Heidi Warriner, Joseph A. Zasadzinski

Research output: Contribution to journalArticlepeer-review

122 Scopus citations

Abstract

Survanta, a clinically used bovine lung surfactant extract, in contact with surfactant in the subphase, shows a coexistence of discrete monolayer islands of solid phase coexisting with continuous multilayer "reservoirs" of fluid phase adjacent to the air-water interface. Exchange between the monolayer, the multilayer reservoir, and the subphase determines surfactant mechanical properties such as the monolayer collapse pressure and surface viscosity by regulating solid-fluid coexistence. Grazing incidence x-ray diffraction shows that the solid phase domains consist of two-dimensional crystals similar to those formed by mixtures of dipalmitoylphosphatidylcholine and palmitic acid. The condensed domains grow as the surface pressure is increased until they coalesce, trapping protrusions of liquid matrix. At ∼40 mN/m, a plateau exists in the isotherm at which the solid phase fraction increases from ∼60 to 90%, at which the surface viscosity diverges. The viscosity is driven by the percolation of the solid phase domains, which depends on the solid phase area fraction of the monolayer. The high viscosity may lead to high monolayer collapse pressures, help prevent atelectasis, and minimize the flow of lung surfactant out of the alveoli due to surface tension gradients.

Original languageEnglish (US)
Pages (from-to)4188-4202
Number of pages15
JournalBiophysical journal
Volume87
Issue number6
DOIs
StatePublished - Dec 2004

Bibliographical note

Funding Information:
Financial support at University of California, Santa Barbara was provided from the National Institutes of Health (grant HL-51177) and the University of California Tobacco Related Disease Research Program (grant 11RT-0222). F.B. acknowledges financial support from the Deutsche Forschungsgemeinschaft (BR 1826/3-4) and beam time at HASYLAB in Hamburg, Germany.

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